Selección de publicaciones más relevantes

Portada de Thromb Haemost 2020;120(2) y del número de Agosto de 2016 de la revista "Proteomics Clinical Applications" (link: http://onlinelibrary.wiley.com/doi/10.1002/prca.201670080/full).

• Artículos:

1. García A, Zitzmann N, Watson SP (2004) Analyzing the platelet proteome. Seminars in Thrombosis and Hemostasis 30:485-489.

2. García A, Prabhakar S, Brock CJ, Pearce AC, Dwek RA, Watson SP, Hebestreit HF, Zitzmann N (2004) Extensive analysis of the human platelet proteome by two-dimensional gel electrophoresis and mass spectrometry. Proteomics 4:656-668.

3. García A, Prabhakar S, Hughan S, Anderson TW, Brock CJ, Pearce AC, Dwek RA, Watson SP, Hebestreit HF, Zitzmann N (2004) Differential proteome analysis of TRAP-activated platelets: involvement of Dok-2 and phosphorylation of RGS proteins. Blood 103:2088-2095.

4. García A, Watson SP, Dwek RA, Zitzmann N. (2005) Applying proteomics technology to platelet research. Mass Spectrometry Reviews 24:918-930.

5. Simmons A, Gangadharan B, Hodges A, Sharrocks K, Prabhakar S, García A, Dwek R, Zitzmann N, McMichael A (2005). Nef mediated lipid raft exclusion of UbcH7 positively regulates T cell signaling. Immunity 23:621-634.

6. Suzuki-Inoue K, Fuller GLJ, García A, Eble JA, Pohlmann S, Inoue O, Gartner TK, Hughan SC, Pearce AC, Schweighoffer E, Zitzmann N, Morita T, Tybulewicz VLJ, Ozaki Y, Watson SP (2006) A novel Syk-dependent mechanism of platelet activation by the C-type lectin receptor CLEC-2. Blood 107:542-549.

7. Senis YA, Tomlinson MG, García A, Dumon S, Heath VL, Herbert J, Cobbold SP, Spalton JC, Ayman S, Zitzmann N, Bicknell R, Frampton J, Authi K, Martin A, Wakelam MJO, Watson SP. (2007) A comprehensive proteomics and genomics analysis reveals novel transmembrane proteins in human platelets and mouse megaka ryocytes including G6b-B, a novel immunoreceptor tyrosine-based inhibitory motif protein. Molecular & Cellular Proteomics 6:548-564.

8. Senis YA, Antrobus R, Severin S, Parguiña AF, Rosa I, Zitzmann N, Watson SP, García A (2009) Proteomic analysis of integrin alphaIIbbeta3 outside-in signaling reveals Src-kinase-independent phosphorylation of Dok-1 and Dok-3 leading to SHIP-1 interactions. Journal of Thrombosis and Haemostasis 7:1718-1726.

9. García A, Senis YA, Antrobus R, Hughes CE, Dwek RA, Watson SP, Zitzmann N. (2006) A global proteomics approach identifies novel phosphorylated signalling proteins in GPVI-activated platelets: involvement of G6f, a novel platelet Grb2-binding membrane adapter. Proteomics 6:5332-5343

10. González-Juanatey JR, Parguiña AF, Rosa I, Grigorian-Shamagián L, Teijeira-Fernández E, Alonso J, Agra R, García A. (2010) Proteomic analysis of circulating human platelets reveals potential biomarkers in non-ST segment elevation acute coronary syndrome. J Am Coll Cardiol. 55;A112.E1047

11. García A (2010) Clinical proteomics in platelet research: challenges ahead. Journal of Thrombosis and Haemostasis 8:1784-1785.

12. García A, Eiras S, Parguiña AF, Alonso J, Rosa I, Salgado-Somoza A, Rico TY, Teijeira-Fernández E, González-Juanatey JR. (2010) High-resolution two-dimensional gel electrophoresis analysis of atrial tissue proteome reveals down-regulation of fibulin-1 in atrial fibrillation. International Journal of Cardiology, 150:283-290.

13. Parguiña AF, Grigorian-Shamajian L, Agra RM, Teijeira-Fernández E, Rosa I, Alonso J, Viñuela-Roldán JE, Seoane A, González-Juanatey JR, García A (2010) Proteins involved in platelet signaling are differentially regulated in Acute Coronary Síndrome: a proteomic study. PLoS One. 2010 Oct 14;5(10) .

14. Parguiña AF, Grigorian-Shamagian L, Agra RM, López-Otero D, Rosa I, Alonso J, Teijeira-Fernández E, González-Juanatey JR, García A. (2011) Variations in Platelet Proteins Associated With ST-Elevation Myocardial Infarction: Novel Clues on Pathways Underlying Platelet Activation in Acute Coronary Syndromes. Arterioscler Thromb Vasc Biol, 31:2957-2964.

15. Parguiña AF, García A.Proteomics applied to the study of platelet-related diseases: Aiding the discovery of novel platelet biomarkers and drug targets. J Proteomics. 2012 Dec 5;76:275-86

16. Shai E, Rosa I, Parguiña AF, Motahedeh S, Varon D, García A. Comparative analysis of platelet-derived microparticles reveals differences in their amount and proteome depending on the platelet stimulus. J Proteomics. 2012 Dec 5;76:287-96.

17. Parguiña AF, Alonso J, Rosa I, Vélez P, González-López MJ, Guitián E, Eble JA, Loza MI, García A. (2012) A detailed proteomic analysis of rhodocytin-activated platelets reveals novel clues on the CLEC-2 signalosome: implications for CLEC-2 signaling regulation.  Blood. 2012;120:e117-126.

18. Senis Y, García A. Platelet proteomics: state of the art and future perspective. Methods Mol Biol. 2012;788:367-99.

19. Ramos A, Rodríguez-Seoane C, Rosa I, Trossbach SV, Ortega-Alonso A, Tomppo L, Ekelund J, Veijola J, Järvelin MR, Alonso J, Veiga S, Sawa A, Hennah W, García A, Korth C, Requena JR. Neuropeptide precursor VGF is genetically associated with social anhedonia and underrepresented in the brain of major mental illness: its downregulation by DISC1. Hum Mol Genet. 2014 Jun 16. pii: ddu303. [Epub ahead of print].

20. Vélez P, Parguiña AF, Ocaranza R, Grigorian L, Rosa I, Alonso-Orgaz S, de la Cuesta F, Guitián E, Moreu J, Barderas MG, González-Juanatey JR, García A. Identification of a circulating microvesicle protein network involved in ST-elevation myocardial infarction. Thromb Haemost. 2014;112:712-726.

22. Vélez P, García A. Platelet proteomics in cardiovascular diseases. Transl Proteomics 2015;7:15-29. doi:10.1016/j.trprot.2014.09.002.

23. Vélez P, Izquierdo I, Rosa I, García, A. A 2D-DIGE-based proteomic analysis reveals differences in the platelet releasate composition when comparing thrombin and collagen stimulations. Sci. Rep. 5, 8198; DOI:10.1038/srep08198 (2015)

24. Ruiz-Riquelme A, Sánchez-Iglesias S, Rábano A, Guillén-Navarro E, Domingo-Jiménez R, Ramos A, Rosa I, Senra A, Nilsson P, García Á, Araújo-Vilar D, Requena JR. Larger aggregates of mutant seipin in Celia's Encephalopathy, a new protein misfolding neurodegenerative disease. Neurobiol Dis. 2015;83:44-53.

25. Vélez P, Ocaranza-Sánchez R, López-Otero D, Grigorian-Shamagian L, Rosa I, Bravo SB, González-Juanatey JR, García A. 2D-DIGE-based proteomic analysis of intracoronary versus peripheral arterial blood platelets from acute myocardial infarction patients: upregulation of platelet activation biomarkers at the culprit site. Proteomics Clin. Appl. 2016, 10, 851–858.

26. García A. Platelet Clinical Proteomics: Facts, challenges and future perspectives. Proteomics Clin Appl. 2016;10:767-773.

27. Izquierdo I, Rosa I, Bravo SB, Guitián E, Pérez-Serra A, Campuzano O, Brugada R, Mangas A, García Á, Toro R. Proteomic identification of putative biomarkers for early detection of sudden cardiac death in a family with a LMNA gene mutation causing dilated cardiomyopathy. J Proteomics. 2016 Oct 4;148:75-84.

28. Vélez P, Ocaranza-Sánchez R, López-Otero D, Grigorian-Shamagian L, Rosa I, Guitián E, García-Acuña JM, González-Juanatey JR, García Á. Alteration of platelet GPVI signaling in ST-elevation myocardial infarction patients demonstrated by a combination of proteomic, biochemical, and functional approaches. Sci Rep. 2016 Dec 22;6:39603. doi: 10.1038/srep39603.

29. Izquierdo I, García A. Platelet proteomics applied to the search for novel antiplatelet therapeutic targets. Expert Rev Proteomics. 2016 Oct 8. [Epub ahead of print] .

30. Akhter S, Chakraborty S, Moutinho D, Álvarez-Coiradas E, Rosa I, Viñuela J, Domínguez E, García A, Requena JR. The human VGF-derived bioactive peptide TLQP-21 binds heat shock 71 kDa protein 8 (HSPA8)on the surface of SH-SY5Y cells. PLoS One. 2017 Sep 21;12(9):e0185176. doi: 10.1371/journal.pone.0185176.

31. Barrachina MN, Calderón-Cruz B, Fernandez-Rocca L, García Á. Application of Extracellular Vesicles Proteomics to Cardiovascular Disease: Guidelines, Data Analysis, and Future Perspectives. Proteomics. 2018 Nov 23:e1800247. doi: 10.1002/pmic.201800247. [Epub ahead of print] Review.

32. Barrachina MN, Sueiro AM, Casas V, Izquierdo I, Hermida-Nogueira L, Guitián E, Casanueva FF, Abián J, Carrascal M, Pardo M, García Á. A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients. Proteomics. 2019 Jan;19(1-2):e1800248. doi: 10.1002/pmic.201800248.

33. Ramos A, Rodríguez-Seoane C, Rosa I, Gorroño-Etxebarria I, Alonso J, Veiga S, Korth C, Kypta RM, García Á, Requena JR. Proteomic Studies Reveal Disrupted in Schizophrenia 1 as a Player in Both Neurodevelopment and Synaptic Function. Int J Mol Sci. 2018 Dec 29;20(1). pii: E119. doi: 10.3390/ijms20010119.

34. Barrachina MN, Sueiro AM, Izquierdo I, Hermida-Nogueira L, Guitián E, Casanueva FF, Farndale RW, Moroi M, Jung SW, Pardo M, García A. GPVI surface expression and signalling pathway activation are increased in platelets from obese patients: Elucidating potential anti-atherothrombotic targets in obesity. Atherosclerosis. 2019 Feb;281:62-70. doi: 10.1016/j.atherosclerosis.2018.12.023.

35. Izquierdo I, Barrachina MN, Hermida-Nogueira L, Casas V, Eble JA, Carrascal M, Abián J, García Á. Platelet membrane lipid rafts protein composition varies following GPVI and CLEC-2 receptors activation. JProteomics. 2019 Jan;19(1-2):e1800248. doi: 10.1002/pmic.201800248. Epub 2018 Dec 27.PMID: 30536591

36. Izquierdo I, Barrachina MN, Hermida-Nogueira L, Casas V, Morán LA, Lacerenza S, Pinto-Llorente R, Eble JA, de Los Ríos V, Domínguez E, Loza MI, Casal JI, Carrascal M, Abián J, García A. A Comprehensive Tyrosine Phosphoproteomic Analysis Reveals Novel Components of the Platelet CLEC-2 Signaling Cascade. Thromb Haemost. 2020 Jan 4. doi: 10.1055/s-0039-3400295. [Epub ahead of print]

37. Hermida-Nogueira L, Barrachina MN, Izquierdo I, García-Vence M, Lacerenza S, Bravo S, Castrillo A, Garcí a Á. Proteomic analysis of extracellular vesicles derived from platelet concentrates treated with Mirasol® identifies biomarkers of platelet storage lesion. J Proteomics. 2020 Jan 6;210:103529. doi: 10.1016/j.jprot.2019.103529. Epub 2019 Oct 9

38. Barrachina MN, Morán LA, Izquierdo I, Casanueva FF, Pardo M, García Á.Sci Rep. (2020) Analysis of platelets from a diet-induced obesity rat model: elucidating platelet dysfunction in obesity. Sci Rep. Aug 4;10(1):13104.

39. Hermida-Nogueira L, Barrachina MN, Morán LA, Bravo S, Diz P, García Á, Blanco J. (2020) Deciphering the secretome of leukocyte-platelet rich fibrin: towards a better understanding of its wound healing properties. Sci Rep. 2020 Sep 3;10(1):14571. doi: 10.1038/s41598-020-71419-7.PMID: 32884030 Free PMC article.

40. Barrachina MN, Hermida-Nogueira L, Moran LA, Casas V, Hicks SM, Sueiro AM, Di Y, Andrews RK, Watson SP, Gardiner EE, Abian J, Carrascal M, Pardo M, García Á (2020) Phosphoproteomic Analysis of Platelets in Severe Obesity Uncovers Platelet Reactivity and Signaling Pathways Alterations. Arterioscler Thromb Vasc Biol. 2020 Nov 5:ATVBAHA120314485. doi: 10.1161/ATVBAHA.120.314485. Online ahead of print.PMID: 33147989.

41. María N. Barrachina,Irene Izquierdo, Lidia Hermida-Nogueira,Luis A. Morán,Amparo Pérez,Ana B. Arroyo,Nuria García-Barberá,Rocío González-Conejero,Sara Troitiño,Johannes A. Eble,José Rivera,Constantino Martínez,María I. Loza,Eduardo Domínguez and Ángel García (2021) The PI3Kd Inhibitor Idelalisib Diminishes Platelet Function and Shows Antithrombotic Potential. Int. J. Mol. Sci. 2021, 22(7), 3304; https://doi.org/10.3390/ijms22073304 (registering DOI).

42. Morán LA, Di Y, Sowa MA, Hermida-Nogueira L, Barrachina MN, Martin E, Mize TH, Clark JC, Eble JA, Moreira D, Pollitt AY, Loza MI, Domínguez E, Watson SP, García A (2021) Katacine is a new ligand of CLEC-2 that acts as a platelet agonist.Thromb Haemost. 2022 Feb 15. doi: 10.1055/a-1772-1069. Online ahead of print.PMID: 35170009.

43.Cheung HYF, Moran LA, Sickmann A, Heemskerk JWM, García A, Watson SP (2022) Inhibition of Src but not Syk causes weak reversal of GPVI-mediated platelet aggregation measured by light transmission aggregometry.Platelets. 2022 May 9:1-8. doi: 10.1080/09537104.2022.2069235. Online ahead of print.PMID: 35535424.

44.Damaskinaki FN, Moran LA, García A, Kellam B, Watson SP (2021) Overcoming challenges in developing small molecule inhibitors for GPVI and CLEC-2.Platelets. 2021 Aug 18;32(6):744-752. doi: 10.1080/09537104.2020.1863939. Epub 2021 Jan 7.PMID: 33406951 Review.

45.Gaëtan Chicanne, Maria N Barrachina, Anaelle Durbec, Justine Bertrand-Michel, Sara Troitiño, Lidia Hermida-Nogueira, Aurelio M Sueiro, María Pardo, Bernard Payrastre, Ángel García (2022) OPlatelet Lipidome Fingerprint: New Assistance to Characterize Platelet Dysfunction in Obesity. Int J Mol Sci.2022 Jul 28;23(15):8326. doi: 10.3390/ijms23158326.

46.Fernández DI, Diender M, Hermida-Nogueira L, Huang J, Veiras S, Henskens YMC, Te Loo MWM, Heemskerk JWM, Kuijpers MJE, García Á (2023) Role of SHP2 (PTPN11) in glycoprotein VI-dependent thrombus formation: Improved platelet responsiveness by the allosteric drug SHP099 in Noonan syndrome patients. Thromb Res. 2023 Jun 5;228:105-116. doi: 10.1016/j.thromres.2023.06.001.

47.Arauna D, Araya-Maturana R, Urra FA, García Á, Palomo I, Fuentes E. Altered dynamics of calcium fluxes and mitochondrial metabolism in platelet activation-related disease and aging. Life Sci. 2024 Aug 15;351:122846. doi: 10.1016/j.lfs.2024.122846. Epub 2024 Jun 14.

48.Martínez-Botía P, Tassi Yunga S, Szklanna P, Babur O, Emili A, Wilmarth PA, Heemskerk JWM, Maguire PB, Iding AFJ, Ramström S, García Á, Aslan JE, Gutiérrez L. Towards standardization and a concerted vision for platelet proteomics research: communication from the ISTH SSC subcommittees Models of Thrombosis and Hemostasis, Platelet Physiology and Predictive and Diagnostic Variables in Thrombotic Disease. J Thromb Haemost. 2025 Feb 12:S1538-7836(25)00064-9. doi: 10.1016/j.jtha.2025.02.002. Online ahead of print.

• Capítulos de libro:

1. García A. Two-dimensional gel electrophoresis in platelet proteomics research. In: Sreejayan, Ren eds. Vascular biology protocols. Volume included in the series “Methods in molecular medicine”. Humana Press, USA, 2007. pp. 339-353.

2. García A, Senis Y, Tomlinson MG, Watson SP. Platelet genomics and proteomics. In: Michelson AD editor. Platelets (second edition). Elsevier/Academic Press, USA, 2007. pp. 99-116.

3. García A, Two-dimensional gel electrophoresis: basic principles and application to platelet signaling studies. In : García A, Senis YA (eds). Platelet proteomics: Principles, analysis and applications. Wiley & Sons, USA, 2011. pp. 89-110.

4. Parguiña AF, García A. Microparticle and exosome proteomic analysis. In: P. Harrison, I. Sargent, C. Gardiner (Eds). Extracellular vesicles in health and disease. 2014; pp. 285-315. Pan Stanford Publishing. USA. ISBN: 978-981-4411-98-1.

4.Barrachina MN, García Á. (2021) Clinical Proteomics for the Analysis of Circulating Extracellular Vesicles. In: Carrera M, Mateos J (eds). Shotgun Proteomics. Methods Mol Biol. 2021;2259:13-23. Humana Press. USA doi: 10.1007/978-1-0716-1178-4_2.

5.Hermida-Nogueira L, Blanco J, García Á. (2023) Secretome Profile of Leukocyte-Platelet-Rich Fibrin (L-PRF) Membranes. In: David W.Greeningand, Richard J. Simpson (eds.). Serum/Plasma Proteomics: Methods and Protocols. Methods Mol Biol. 2023;2628:207-219. doi: 10.1007/978-1-0716-2978-9_14.

• Editor de libro:

1. Editor del libro “Platelet proteomics: Principles, analysis and applications”. Ángel García, Yotis A Senis (eds). Wiley & Sons, USA, 2011.