ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Student's work ECTS: 51 Hours of tutorials: 3 Expository Class: 9 Interactive Classroom: 12 Total: 75
Use languages Spanish, Galician
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology
Areas: Biochemistry and Molecular Biology
Center Faculty of Medicine and Dentistry
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable | 1st year (Yes)
Provide students with a comprehensive review of the underlying concepts, history, issues addressed at present and its projection into the future of molecular biology. We will study the anatomy of the genome and chromatin structure, and analyze the basic processes of DNA replication, repair and recombination, as well as transcription and translation. Analyze the mechanisms of folding, modification, trafficking and degradation of proteins. Review current knowledge of the structure and organization of the human genome from the chromosome to the nucleotide level. Special emphasis will be on the comparison between genomes of different species as a strategy for studying the evolution, structure and function of genes and noncoding sequences of the human genome and model organisms. It will be introduced to the management of experimental tools in molecular biology.
PART 1. DNA: STRUCTURE, REPLICATION AND REPAIR
1. DNA and the nucleus. Deep sequencing. Structure of genes and genomes. Histones. Levels of DNA packaging. Covalent modification of histones and histone subtypes. Current models of chromatin structure. Different kinds of chromatin structure: molecular basis of its establishment. ENCODE Project: techniques for functional genomics.
2. DNA replication. Molecular mechanism of replication. Enzymatic machinery involved in replication. Replication initiation. Regulation of the stability of the replication fork. Chromatin and Replication. Replisomes. Election of replication origins: “dormant” origins. Molecular combing and other techniques to study DNA replication.
3. DNA repair and recombination. DNA lesions. Types of repair. Repair of double-strand breaks by homologous recombination. Homologous recombination: general mechanisms and its applications in the cell. Transposition and conservative sequence specific recombination.
PART 2. GENE EXPRESSION
1. Transcription. Introducing a eukaryotic gene expression. Transcription process and its components. Synthesis of hnRNAs. Maturation, transport and localization of mRNAs. The process of translation and its components. Folding, modification, trafficking and degradation of proteins.
2. Regulation of transcription. Introduction to the regulation of gene expression and control points. Chromatin structure and transcriptional activity. Regulatory elements and transcription factors. Mechanisms of transcriptional regulation. Particular cases of transcriptional control.
3. Post-transcriptional regulation. Pretranslational Control. Eukaryotic translation regulation. The interferon system as an example of integrated regulation of gene expression.
- ALBERTS, B., A. JOHNSON, J. LEWIS, M. RAFF, 2008 THE MOLECULAR BIOLOGY OF THE CELL GARLAND PUBLISHING.
- LEWIN, B. 2008 Genes IX. Oxford University Press
- PAGEL and A. POMIANKOWSKI (Ed.), 2008 Evolutionary genomics and proteomics. Sinauer Associates Inc., Sunderland, MA.
- WEAVER, R. F., 2008 Molecular Biology.McGraw-Hill International Edition
(Relevant scientific papers for the different topics of the matter will also be used)
1. To be able to make judgments about hypotheses, experimental approaches and experiments in the field of Molecular Biology
2. To become familiar with relevant techniques in the fields of Molecular Biology
3. To design experiments in these fields in order to answer relevant questions
4. Understand the fundamentals of research in molecular biology
5. Provide the main strategies for the identification of genome-wide functional motifs
The teaching methodology to be applied:
1. Theoretical classes.
2. Seminars. Collective discussion of a selection of scientific articles relevant to the topics of the course proposed by students and teachers, who will present them individually.
For cases of fraudulent performance of exercises or tests, the provisions of the “Regulations for the evaluation of students academic performance and qualifications review” will apply.
1. A final exam of short questions might be done: giving the student the opportunity to demonstrate the conceptual domain acquired (up to 5 points)
2. Presentation of a scientific paper: the understanding of the study and the clarity of exposition will be taken into account (up to 8 points depending on the realization of the final exam.)
3. Active participation in class with questions, comments and opinions will be valued up to 2 points.
All exams and tests will be face-to-face.
Lectures: 7 (T), 14 (S), 1 (Tut), 4 (exam)
Homework:21 (T), 28 (S)
TOTAL: 26 (lectures) +49 (homework)= 75
T= theory
S= seminars
Tut= tutorials
Assistance to classes and seminars
The three official languages of the course are Galician, Spanish and English. It is up to the teacher's authority indiscriminate use of either.
Jose Manuel Martinez Costas
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881815734
- jose.martinez.costas [at] usc.es
- Category
- Professor: University Professor
| Monday | |||
|---|---|---|---|
| 10:30-11:30 | Grupo /CLE_01 | Spanish | Aulario-Classroom 10 |
| Wednesday | |||
| 09:30-11:30 | Grupo /CLE_01 | Spanish | Aulario-Classroom 10 |