ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Student's work ECTS: 99 Hours of tutorials: 3 Expository Class: 24 Interactive Classroom: 24 Total: 150
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Chemistry Engineering
Areas: Chemical Engineering
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
The bioreactor is the main nucleus of biotechnological processes on an industrial scale, which presents specific and differentiated characteristics from the reactors of the process industry. The general objective of the course is to introduce the bases of design and operation of bioreactors.
The contents are structured in the following topics:
UNIT 1: Introduction (5h: 3hE +2hTG)
Objective: Introduction to the basic aspects of bioreactors and presentation of conventional configurations and other bioreactor models particularly adapted to increase the efficiency of certain bioprocesses. Description of industrial processes. Flow diagrams.
Contents:
1.1 Basic aspects of bioreactors: objectives and factors limiting conversion; classification
1.2 Bioreactors with solid substrates: stacks, trays, drum, fixed bed, fluidized bed
1.3 Liquid phase bioreactors: conventional/non-conventional configurations (airlift, bioreactors with cell retention, bioreactors with product separation, photobioreactors...)
UNIT 2: Enzymatic and microbial kinetics (14.5h: 9hE+3hS+2,5I)
Objective: Description of the fundamental concepts of enzymatic and microbial catalysis, and speed equations that describe these processes. Methods of immobilization and possible effects on the transfer of matter.
Contents:
2.1 Enzyme kinetics: single substrate reactions; inhibition reactions; variation of activity with temperature and pH
2.2 Microbial kinetics: growth requirements and formulation of culture medium; stoichiometry; yields; cell growth and kinetics; models
2.3 Immobilization of biocatalysts: types; kinetics of immobilized biocatalysts
UNIT 3: Designing ideal bioreactors (16h: 7hE+4hS+5hI)
Objective: Analysis of conventional bioreactors, assuming simple kinetics and ideal hydraulic behaviour, to obtain applicable mathematical equations for bioreactor design and operation.
Contents:
3.1 Mass balances applied to bioreactors
3.2 Stirred tank reactor: discontinuous, fed-batch, continuous, in series, cell recirculation
3.3 Piston Flow Reactor: Cell Recirculation
UNIT 4: Design of real bioreactors (12h: 6hE+4hS+2hI)
Objective: Study of the deviations from ideal hydrodynamic behaviour and effect on the conversion and performance of the bioprocess. Oxygen supply in aerobic cultures, agitation in complete mix bioreactors and sterilization processes. Aspects associated with the change of scale. Description of the characteristics and operation of the main non-conventional reactors.
Contents:
4.1 Non-ideal flow: DTR, flow models, determination of mixing time
4.2 Aeration: Determination of kLa and dependence on operational parameters
4.3 Agitation: power consumption; agitation in aerated systems
4.4 Sterilization: heat treatment (kinetics and effect of temperature); sterilization systems in practice; other inactivation treatments
4.5 Change of scale: concepts, most common criteria
UNIT 5. Bioseparations (2.5h: 2hE+0.5hS)
Elimination of biomass. Cellular disruption. Membrane-based techniques. Extraction. Adsorption and Chromatography.
Basic
Biochemical Engineering and Biotechnology (2nd Edition) [0-444-63357-X]. Najafpour, Ghasem
Elsevier ScienceDirect Books Complete. The students have access to this volume
Bailey, J.E., e Ollis, D.F. Biochemical Engineering Fundamentals. 2nd ed. McGraw Hill, New York (1986).
Additional
Aiba, S., et al. Biochemical Engineering. 2nd ed. University of Tokyo Press, Tokyo (1973).
Atkinson, B., e Mavituna, F. Biochemical Engineering and Biotechnology Handbook. Stockton Press (1991).
Jagnow, G., e David, W. Biotecnología. Introducción con experimentos modelo. Ed. Acribia (1991).
Gòdia Casablancas, F., e López Santín, J. Ingeniería Bioquímica. Ed. Síntesis. Madrid (1998).
Moo-Young, M. Comprehensive Biotechnology. Pergamon Press (1985)
Rem, H., e Reed, G. Biotechnology. Verlag CEIME (1995)
Stroev, E.A., e Makarova, V.G. Laboratory Manual in Biochemistry. MIR (1989)
Wiseman, A. Handbook of Enzyme Biotechnology. Ellis Horwood (1985).
The training activities and the competences that will be worked on in each of them are described below.
Exhibition classes Exhibition of contents with the support of audiovisual media.
Resolution of a standard exercise on a blackboard.
Use of the virtual campus.
Periodic test (online tool).
CG1;CG5;CB1;CB3;CB4;CB5;CT6;CE6
Interactive classes and seminars
Resolution of exercises by students individually or in small groups (2-3).
Use of spreadsheet.
Delivery of one exercise per bulletin.
CG3;CG4;CG5;CB1;CB2;CB4;CT1;CT6;CE6
Interactive computer classes Kinetics resolution. Introduction to the use of the Superpro Designer simulator and practical exercises.
Delivery of an exercise.
CG2;CB1;CB3;CT1;CE6
Group tutoring Group work (2-3) on non-conventional bioreactor.
Poster presentation.
CG3;CG4;CG5;CB1;CB2;CB3;CB4;CT2*;CT3*;CT4*;CE6
Teaching methodology in Scenario 1:
Theory classes will be alternated with seminars in which problems applied to real cases will be evaluated. The basic theoretical contents of the subject will be taught on the basis of lectures where they will be explained and developed. These classes will be supported by the use of Power Point presentations available in Virtual Campus. The calculation sheet used for problem solving will be mainly Excel. The group tutorials will focus on the study in greater detail of different types of bioreactors or biotechnological processes through the use of on-line bibliographical resources in the classroom.
Teaching methodology in Scenario 2:
Lectures will be held in Teams.
The interactive teaching sessions (seminars) and computer practices will be carried out in the classroom if the number of students allows it, and if not, they will be alternated with virtual classes in Teams.
Teaching methodology in Scenario 3
Lectures, interactive and laboratory teaching sessions will be held in Teams.
In the final phase of the teaching stage, we will try to motivate the student by proposing the application of the knowledge acquired to a specific example referring to an installation or industrial product. Each student will present and defend the solutions proposed in each case in a classroom session (Scenario 1) or through the Teams platform (Scenarios 2 and 3).
The language of instruction will be Spanish
A continuous evaluation of the learning process will be carried out, through the assignment of 6 short assignments: short reports (3) and problem solving (3). This continuous evaluation will be the basis for a first grade, which will count for 60% of the final grade, 10% for each submission. The work developed in the group workshops will also be evaluated and the corresponding grade will account for 10% of the final grade. At the end of the classes there will be a theoretical and practical exam, including theoretical questions and problem solving, which will count for 30% of the final grade.
Evaluation of the Exam in Scenario 1: Classroom Exam
Evaluation of the test in Scenarios 2 and 3: Exam through the Virtual Campus or Teams platforms
At the first and second opportunity the evaluation is the same and the grades are maintained. In case of fraudulent exercises or tests, the provisions of the Regulations for the Evaluation of Students' Academic Performance and for the Review of Grades shall apply.
The course has a workload equivalent to 6 ECTS which are distributed as described below.
Activities Attendance hours Non-attendance hours
Lectures 27 45
Interactive classes seminars 11 22
Interactive computer classes 10 10
Tutoring group 2 4
Individualized Tutoring 1 2
Examination and review 4 12
Total 55 95
It is recommended that the student has previously passed the subjects Fundamentals of Biological Processes, Thermodynamics and Chemical Kinetics and Biochemistry I.
The course will be taught in Spanish.
The use of the virtual campus as well as MS Teams is necessary.
Compliance with safety measures in the classroom, interactive and laboratory sessions.
Recommendations for telematic teaching:
- It is necessary to have a computer with a microphone and a camera to carry out the telematic activities that are programmed during the course. It is recommended to acquire equipment with MS Windows environment, since other platforms do not support some of the software used in the subjects, available at the USC.
- Improve your information and digital skills with resources available at the USC.
Follow scrupulously all the indications of the health authorities and of the USC, for the protection of the health of Covid-19. Wear a mask, apply hydroxel or wash your hands with water and soap as indicated and, whenever possible, increase the distance between you and your classmates and teacher in the classroom.
CONTINGENCY PLAN
A) Teaching methodologies
Teaching development in Scenario 2:
Lectures will be held in Teams.
The interactive and laboratory teaching sessions will be held in the classroom.
Development of teaching in Scenario 3:
Lectures, interactive and laboratory teaching sessions will be held in Teams.
B) Evaluation systems
Evaluation of the test in Scenarios 2 and 3: Exam through the Virtual campus or Teams platforms
Maria Teresa Moreira Vilar
Coordinador/a- Department
- Chemistry Engineering
- Area
- Chemical Engineering
- Phone
- 881816792
- maite.moreira [at] usc.es
- Category
- Professor: University Professor
Miguel Mauricio Iglesias
- Department
- Chemistry Engineering
- Area
- Chemical Engineering
- Phone
- 881816800
- miguel.mauricio [at] usc.es
- Category
- Professor: LOU (Organic Law for Universities) PhD Assistant Professor
| Monday | |||
|---|---|---|---|
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
| Tuesday | |||
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
| 05.24.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 05.24.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
| 07.12.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 07.12.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |