ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Student's work ECTS: 99 Hours of tutorials: 3 Expository Class: 24 Interactive Classroom: 24 Total: 150
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Chemistry Engineering
Areas: Chemical Engineering
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
The bioreactor is the core of biotechnological processes on an industrial scale, which presents specific and differentiated characteristics from the reactors of the process industry. The general objective of the course is to introduce the bases of design and operation of bioreactors.
The contents of the course, according to the degree syllabus, are the following:
- Stoichiometry. Enzyme and microbial kinetics. Rate equations and kinetic models.
- Ideal bioreactor design equations: batch reactor, CSTR and PFR
- Multiple and recirculating bioreactor systems.
- Non-ideal flow.
- Stirring and aeration.
- Sterilisation: kinetics and equipment.
Seminars
- Solving bioreactor design and operation problems using a spreadsheet.
Computer room
- Use of the process simulator in the design and operation of bioreactors.
The contents are structured in the following units:
UNIT 1: Introduction (5h: 3hE +2hTG)
Objective: Biotechnological processes and study of conventional configurations and other bioreactor models. Description of industrial processes.
Contents:
1.1 Basic aspects of bioreactors: objectives and factors limiting conversion; classification
1.2 Bioreactors with solid substrates: stacks, trays, drum, fixed bed, fluidized bed
1.3 Liquid phase bioreactors: conventional/non-conventional configurations (airlift, bioreactors with cell retention, bioreactors with product separation, photobioreactors...)
UNIT 2: Enzymatic and microbial kinetics (14.5h: 9hE+3hS+2,5I)
Objective: Description of the fundamental concepts of enzymatic and microbial catalysis, and speed equations that describe these processes. Methods of immobilization and possible effects on the transfer of matter.
Contents:
2.1 Enzyme kinetics: single substrate reactions; inhibition reactions; variation of activity with temperature and pH
2.2 Microbial kinetics: growth requirements and formulation of culture medium; stoichiometry; yields; cell growth and kinetics; models
2.3 Immobilization of biocatalysts: types; kinetics of immobilized biocatalysts
UNIT 3: Designing ideal bioreactors (16h: 7hE+4hS+5hI)
Objective: Analysis of conventional bioreactors, assuming simple kinetics and ideal hydraulic behaviour, to obtain applicable mathematical equations for bioreactor design and operation.
Contents:
3.1 Mass balances applied to bioreactors
3.2 Stirred tank reactor: discontinuous, fed-batch, continuous, in series, cell recirculation
3.3 Piston Flow Reactor: Cell Recirculation
UNIT 4: Design of real bioreactors (12h: 6hE+4hS+2hI)
Objective: Study of the deviations from ideal hydrodynamic behaviour and effect on the conversion and performance of the bioprocess. Oxygen supply in aerobic cultures, agitation in complete mix bioreactors and sterilization processes. Aspects associated with the change of scale. Description of the characteristics and operation of the main non-conventional reactors.
Contents:
4.1 Non-ideal hydrodynamic behaviour, flow models, mixing time
4.1 Aeration: Determination of kLa and dependence on operational parameters
4.2 Agitation: power consumption; agitation in aerated systems
4.3 Sterilization: heat treatment (kinetics and effect of temperature); sterilization systems in practice; other inactivation treatments
UNIT 5. Bioseparations (2.5h: 2hE+0.5hS)
Elimination of biomass. Cellular disruption. Membrane-based techniques. Chromatography and other purification units
Basic
Biochemical Engineering and Biotechnology (2nd Edition) [0-444-63357-X]. Najafpour, Ghasem
Elsevier ScienceDirect Books Complete. The students have access to this volume
Bailey, J.E., e Ollis, D.F. Biochemical Engineering Fundamentals. 2nd ed. McGraw Hill, New York (1986).
Additional
Aiba, S., et al. Biochemical Engineering. 2nd ed. University of Tokyo Press, Tokyo (1973).
Atkinson, B., e Mavituna, F. Biochemical Engineering and Biotechnology Handbook. Stockton Press (1991).
Jagnow, G., e David, W. Biotecnología. Introducción con experimentos modelo. Ed. Acribia (1991).
Gòdia Casablancas, F., e López Santín, J. Ingeniería Bioquímica. Ed. Síntesis. Madrid (1998).
Moo-Young, M. Comprehensive Biotechnology. Pergamon Press (1985)
Rem, H., e Reed, G. Biotechnology. Verlag CEIME (1995)
Stroev, E.A., e Makarova, V.G. Laboratory Manual in Biochemistry. MIR (1989)
Wiseman, A. Handbook of Enzyme Biotechnology. Ellis Horwood (1985).
The training activities and the competences that will be worked on in each of them are described below.
Exhibition classes Exhibition of contents with the support of audiovisual media.
Resolution of a standard exercise on a blackboard.
Use of the Moodle learning management system (Campus Virtual)
CG1;CG5;CB1;CB3;CB4;CB5;CT6;CE6
Interactive classes and seminars
Resolution of exercises by students individually or in small groups (2-3).
Use of spreadsheet.
Delivery of one exercise per bulletin.
CG3;CG4;CG5;CB1;CB2;CB4;CT1;CT6;CE6
Interactive computer classes Kinetics resolution. Use of the Superpro Designer software for bioreactor design.
Delivery of an exercise.
CG2;CB1;CB3;CT1;CE6
Group tutoring Group work (2-3) on non-conventional bioreactor.
Poster presentation.
CG3;CG4;CG5;CB1;CB2;CB3;CB4;CT2*;CT3*;CT4*;CE6
Theory classes will be alternated with seminars in which problems applied to real cases will be evaluated. The basic theoretical contents of the subject will be taught on the basis of lectures where they will be explained and developed. These classes will be supported by the use of Power Point presentations available at the Moodle Learning Management System (Campus Virtual). The calculation sheet used for problem solving will be mainly Excel. The group tutorials will focus on the study in greater detail of different types of bioreactors or biotechnological processes through the use of on-line bibliographical resources in the classroom.
The language of instruction will be Spanish
A continuous evaluation of the learning process will be carried out, through the assignment of 2 problem solving-exercises (in problem seminars or computer seminar). This continuous evaluation will count for 30% of the final grade. At the end of the classes there will be a theoretical and practical exam, including theoretical questions and problem solving, which will count for 70% of the final grade.
At the first and second opportunity the evaluation is the same and the grades are maintained. In case of fraudulent exercises or tests, the provisions of the Regulations for the Evaluation of Students' Academic Performance and for the Review of Grades shall apply.
A student will be graded as "No show" if s/he does not attend to the final exam
The different skills are assessed in the activities as follows:
Final exam: CG1; CG5; CB1; CB3; CB4; CB5; CT6; CE6
Handed-in problems CG3; CG4; CG5; CB1; CB2; CB4; CT1; CT6; CE6
Process simulator assignment CG2; CB1; CB3; CT1; CE6
Group tutorial, incluing group assignment and presentation: CG3; CG4; CG5; CB1; CB2; CB3; CB4; CT2 *; CT3 *; CT4 *; CE6
The course has a workload equivalent to 6 ECTS which are distributed as described below.
Activities Attendance hours Non-attendance hours
Lectures 27 45
Interactive classes seminars 11 22
Interactive computer classes 10 10
Tutoring group 2 4
Individualized Tutoring 1 2
Examination and review 4 12
Total 55 95
It is recommended that the student has previously passed the subjects Fundamentals of Biological Processes, Thermodynamics and Chemical Kinetics and Biochemistry I.
For cases of fraudulent performance of exercises or tests, the provisions of the "Regulations for the evaluation of students' academic performance and review of qualifications" shall apply.
The course will be taught in Spanish.
The use of the Moodle Learning Management System (Campus Virtual) as well as MS Teams is necessary.
Compliance with safety measures in the classroom, interactive and laboratory sessions.
Recommendations for telematic teaching:
- It is recommended to have a computer with a microphone and a camera to carry out the telematic activities that are programmed during the course. It is recommended to acquire equipment with MS Windows environment, since other platforms do not support some of the software used in the subjects, available at the USC.
- Improve your information and digital skills with resources available at the USC.
The version in Galician of this guide will prevail over the Spanish and English version in case of conflict among them.
Maria Teresa Moreira Vilar
Coordinador/a- Department
- Chemistry Engineering
- Area
- Chemical Engineering
- Phone
- 881816792
- maite.moreira [at] usc.es
- Category
- Professor: University Professor
Miguel Mauricio Iglesias
- Department
- Chemistry Engineering
- Area
- Chemical Engineering
- Phone
- 881816800
- miguel.mauricio [at] usc.es
- Category
- Professor: LOU (Organic Law for Universities) PhD Assistant Professor
Daniel Sastre Quemada
- Department
- Chemistry Engineering
- Area
- Chemical Engineering
- Phone
- 881816771
- daniel.sastre [at] usc.es
- Category
- Researcher: Juan de la Cierva Programme
| Monday | |||
|---|---|---|---|
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| Tuesday | |||
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| 06.02.2023 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 07.13.2023 10:00-14:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |