ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 76.5 Hours of tutorials: 4.5 Expository Class: 13.5 Interactive Classroom: 18 Total: 112.5
Use languages Spanish, Galician
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology
Areas: Biochemistry and Molecular Biology
Center Higher Technical Engineering School
Call: First Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable | 1st year (Yes)
To provide knowledge of biology, especially of biochemistry and molecular biology, by means of procedures of study, experience and practice, and encouraging critical thinking, in order that the futures Master in Chemical Engineering and Bioprocess could develop their professional skills related to the biotechnological area.
UNIT 1. General introduction to Biochemistry and Molecular Biology. Estructure and function of biomolecules. Transduction and energy storage. Metabolism overview.
UNIT 2. Introduction to the metabolism. Concept of metabolism. Types of routes and metabolic reactions. Bioenergetics.
UNIT 3. Enzymes. Characteristics of the enzymatic reaction. Kinetic Mm. Enzyme inhibition. Concept of alosterism. Regulation of the enzymatic activity.
UNIT 4. Central metabolic pathways and energetic metabolism. Example: bioethanol production in Saccharomyces cerevesiae and Zymomonas mobilis. Brief summary of other metabolic pathways and their integration in the central pathways.
UNIT 5. Metabolism integration. Integration of metabolic pathways. Production of secondary products. Regulation of metabolism.
UNIT 6. Central Dogma of Molecular Biology. DNA structure. Genes and Genomes. DNA expression: transcription and translation. Genetic Code and mutations.
UNIT 7. Regulation of Gene Expression. Regulation of gene expression in prokaryotes: the lac operon. Regulation of gene expression in eukaryotes.
UNIT 8. Genetic engineering. Manipulation and analysis of DNA molecules. DNA sequencing. PCR. Cloning. Vector types. Transforming prokaryotic and eukaryotic cells.
UNIT 9. Applications of Bioengineering. Genetically modified organisms. Production of recombinant proteins. Examples of Metabolic engineering.
Students will have available (both in the laboratory and in the practice manual) all the information about the potential risks of the practices (handling and disposal of biological material, flammable and / or toxic products, materials at high temperatures) and necessary PPE (lab coat, safety goggles, chemical and thermal protection gloves).
PRACTICE 1: Cloning of a cDNA. Bacterial transformation by heat shock with a recombinant plasmid. Plasmid isolation from bacterial cultures by the alkaline lysis method. Digesting the plasmid with restriction enzymes. PCR amplification of cDNA.
PRACTICE 2: Analysis of amplified cDNA. Analysis by agarose gel electrophoresis of the purified plasmid and digested with restriction enzymes and the products of the PCR reaction. CDNA purification from agarose gel. Growth in liquid medium transformed colonies.
PRACTICE 3: Expression and purification of recombinant protein. IPTG induction of recombinant protein expression in transformed cells. Extraction and purification of the recombinant protein by affinity chromatography.
PRACTICE 4: Analysis of the recombinant protein by electrophoresis.
Preparation of polyacrylamide gels. SDS-PAGE electrophoresis for determining the molecular weight of the recombinant protein.
BASIC BIBLIOGRAPHY
CLARK, D.P. and PAZDERNIK, N.J., 2016. Biotechnology. [en liña] 2nd ed. Amsterdam: Elsevier Academic Press. Dispoñible en: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
McKEE, T. and McKEE, J. R., 2014. Bioquímica. Las bases moleculares de la vida. [en liña] 5 ed. McGRAW-HILL Interamericana Editores. https://accessmedicina-mhmedical-com.ezbusc.usc.gal/book.aspx?bookid=19…
COMPLEMENTARY BIBLIOGRAPHY
FEDUCHI CANOSA, E. e outros. Bioquímica. Conceptos esenciales. 2ª edición. Madrid: Editorial Panamericana, 2015. ISBN: 978-84-9835-875-9
GLICK, B. R., J.J PASTERNAK and C.L. PATTEN, C. L. Molecular biotechnology: principles and applications of recombinant DNA. 4ª edición. Washington D.C; ASM Press, 2010. ISBN: 978-1-55581-498-4
HERRÁEZ SÁNCHEZ, Á. Texto ilustrado e interactivo de biología molecular e ingeniería genética: conceptos, técnicas y aplicaciones en ciencias de la salud. 2ª edición. Barcelona: Elsevier, 2012. ISBN: 978-84-8086-647-7
NELSON, D. L. and M.M. COX. Lehninger Principios de Bioquímica. 6ª edición. Barcelona: Editorial Omega, 2014. ISBN: 978-84-282-1603-6
TYMOCZKO, J. L., J.M. BERG and L. STRYER. Bioquímica: Curso básico. 2ª edición. Barcelona: Editorial Reverté, 2014. ISBN:788429176032
OKAFOR, N. Modern industrial microbiology and biotechnology. Enfield (NH): Science Publishers, 2007. ISBN: 1578084342, 9781578084340
To help meet the skills included in the degree repor tCB6, CB7, CB10, CG7, CE1, CE3, CT4 y CT5. To a greater extent the skills CG7 and CE3.
- Lectures: Lesson taught by the teacher who may have different formats (theory, problems and / or general examples, general guidelines of matter ...). The teacher may have the support of audiovisual and computer but in general, students do not need to handle them in class. Skills: CE1, CB6 and CB10.
- Interactive Classes (Schools): The aim of the seminars is to support the theory and practical classes. Students will conduct an essay coordinated between the three subjects of Module I, which will address a topic related to Bioprocesses from three perspectives, and they will have to present in writing and orally. Skills: CE1/3, CG7, CB7 and CT4.
- laboratory practical classes: This includes classes that take place in a laboratory. The student will have a laboratory manual, including general considerations on the laboratory work and a script for each of the practical activities, which consist of a brief presentation of the rationale, methodology to be followed and indication of the calculations to be performed and results to be presented.
The delivery of a final report is required for the evaluation and shall be delivered to the end of practice. Skills: CG7, CT4 and CT5.
- Tutoring: clarification of doubts about the theory or practice.
Scenario 1. Adapted normality
- MASTER LECTURES face-to-face with computer projections and course in the on-line Campus (including material related to the classes and questionnaires).
- SEMINARS face-to-face in small groups, where problems or issues will be solved. The students will also be able to prepare and discuss topics related to the subject.
- TUTORIALS in very small groups for the clarification of doubts about the theory or practices, to provide information or guide the students. To be held electronically.
- LABORATORY PRACTICAL CLASSES, face-to-face (in small groups) in which the students, following the protocols prepared for this purpose, will handle the appropriate equipment and solve practical questions.
- Among the activities corresponding to this Module, a visit will be made to a bioethanol production plant, which will allow them to understand the industrial application of the theoretical knowledge explained about this process, and which will be evaluated by the final exam.
Scenario 2. Distancing
- MASTER LECTURES AND TUTORIALS will be synchronous, by distance learning using Microsoft Teams.
- SEMINARS face-to-face in small groups.
- LABORATORY PRACTICAL CLASSES, face-to-face in very small groups. If necessary, 50% of the hours will consist of distance learning activities, non-synchronous using the Campus Virtual.
There will be no visit to a company, so this activity will be replaced by a video or explanatory talk.
Scenario 3. Closure of facilities.
All teaching activities (master classes, seminars, tutorials and practical classes) will be held by distance learning, in a synchronous way, using the on-line Campus and Microsoft Teams platform.
There will be no visit to a company, so this activity will be replaced by a video or explanatory talk.
The evaluation will consist of two parts:
1.1) Continuous assessment (50% of the final grade), which in turn consists of:
i. Seminars: oral and written presentation of work (20%).CE1, CE3,CG7, CB7, CT4..
ii. Laboratory Practice: laboratory work and final report (25%). CG7, CT4, CT5.
iii.Participation/ Teacher Report (5%).
Attendance to laboratory practical classes is mandatory. Absences must be properly justified.
The control of attendance to face-to-face activities will be by signature. Participation in telematic activities will be automatically registered in the on-line Campus and Microsoft Teams.
In scenario 1 and 2 evaluation will be face-to-face, in scenario 3 by electronic means (Campus Virtual and Microsoft Teams).
1.2) Final Test (50% of the final grade): it is necessary to pass this exam to pass the subject (50%). CE1, CB6, CB10.
In scenario 1 it will be face-to-face, in scenarios 2 and 3 it will be synchronous telematics.
Students who fail the course in the first opportunity may retake the final exam during the second opportunity, maintaining the scores obtained for the continuous evaluation.
The repeaters are required to take the whole course again.
In cases of fraudulent carrying out of exercises or tests, the provisions of the "Student Assessment and Academic Performance Assessment Regulations" of the USC will apply.
Activity: Attendance hours/ Individual work /ECTS
Theoretical classes: 15 / 15/ 1,2
Seminars: 8/ 16,5/ 0,98
Laboratory sessions: 18/ 20/ 1,52
Group tutorships: 1/ 4/ 0,2
Individual tutorships: 1/ 4/ 0,2
Exam and revision: 2/ 8/ 0,4
Total: 45/ 67,5/ 4,5
The student must revise and extend the theoretical concepts introduced in the different subjects using the recommended text books. Students who encounter significant difficulties when working the proposed activities must come to the tutorials of the teacher, in order that it can analyze the problem and help resolve these difficulties.
Classes are taught in Spanish.
Students will find additional material related to the classes in the "Campus Virtual".
In the subjects for this module (Bioengineering, Biocatalysis and Bioprocessing) the bioethanol production process will be studied in a coordinated manner addressing this topic from a different perspective.
For the admission and permanence of the registered student in the laboratory for practical classes, is mandatory that they know the information and comply with the rules included in the General Safety Standards for experimental spaces of the “Escola Técnica Superior de Enxeñaría”, available in the security section of its website.
Contingency plan
Adaptations for scenarios 2 and 3 are as follows:
Teaching methodology
Scenario 2. Distancing
- MASTER LECTURES will be synchronous, by distance learning using Microsoft Teams plattform.
- SEMINARS face-to-face in small groups.
- TUTORIALS in very small groups to be held electronically and synchronously.
- LABORATORY PRACTICAL CLASSES, face-to-face in very small groups. If necessary, 50% of the hours will consist of explanatory videos of analysis techniques, and interpretation of the results of the experiments.
There will be no visit to a company, so this activity will be replaced by a video or explanatory talk.
Scenario 3. Closure of facilities.
All teaching activities (master classes, seminars, tutorials and practical classes) will be held by distance learning, using the on-line Campus and Microsoft Teams platform.
There will be no visit to a company, so this activity will be replaced by a video or explanatory talk.
Assessment system
The same evaluation system will be maintained for all three scenarios, except that all tests in Scenario 3 (and the final test in Scenario 2) will be conducted electronically.
Jaime Jose Gomez Marquez
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816927
- jaime.gomez.marquez [at] usc.es
- Category
- Professor: University Professor
Cristina Diaz Jullien
Coordinador/a- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816932
- cristina.diaz [at] usc.es
- Category
- Professor: Temporary PhD professor
| Wednesday | |||
|---|---|---|---|
| 10:00-12:00 | Grupo /CLE_01 | Spanish | Classroom A5 |
| 10.30.2020 09:00-14:00 | Grupo /CLIS_01 | Classroom A6 |
| 10.30.2020 09:00-14:00 | Grupo /CLE_01 | Classroom A6 |
| 10.30.2020 09:00-14:00 | Grupo /CLIL_01 | Classroom A6 |
| 06.22.2021 10:00-14:00 | Grupo /CLIL_01 | Classroom A8 |
| 06.22.2021 10:00-14:00 | Grupo /CLIS_01 | Classroom A8 |
| 06.22.2021 10:00-14:00 | Grupo /CLE_01 | Classroom A8 |