Kinetic analysis is a basic tool for the elucidation of reaction mechanisms. The main experimental techniques we use to monitor reaction progress are indicated below. All the instruments that we use are available in our lab, except the NMR instrument, that belongs to the research infrastructure network (RIAIDT) at USC.

UV-Vis spectroscopy

Varian Cary 100 BIO UV-Vis absorption spectrophotometer.

Varian Cary 50 UV-Vis absorption spectrophotometer.

NMR spectroscopy

Varian Inova 500 MHz NMR spectrometer, located at the Center for Research in Biological Chemistry and Molecular Materials (CIQUS).

High performance liquid chromatography (HPLC)

Waters 2695XE HPLC system equipped with Waters 2996 photodiode array detector.

Rapid mixing techniques

The kinetic study of reactions with a half-life of less than a few seconds requires the use of rapid mixing methods. When this is the case, we use stopped-flow instruments.

Applied Photophysics SX17.MV Stopped Flow Spectrometer (upgraded recently). Applied Photophysics RX2000 Rapid Mixing Stopped-Flow Unit.