In our lab we use several fluorescence spectroscopic techniques:

• Steady state and time resolved fluorescence spectroscopy
• Fluorescence Correlation Spectroscopy (FCS) on free diffusing molecules (home-built)
• Single Molecule Fluorescence Detection (SMFD)
• Single-Molecule Fluorescence Imaging with Total Internal Reflection Microscopy (sm-TIRF) of immobilized molecules (home-built)

Data-analysis

Rigorous data-analysis is essential for the complex systems we study.
We use mostly home-written software coded in Matlab, Mathematica or Origin.

• Principal Component Global Analysis (PCGA)
• Concentration model for the precise determination of critical micelle concentrations