Lecture: «Advanced homo-FRET modelling in biophysics – Exact analytical solutions on structural studies of the KcsA channel»
Prof. Manuel Prieto (CQFM and IN, Instituto Superior Técnico, Universidade de Lisboa)
CiQUS Seminar Room - 12:15h
Donor decay ensemble average in FRET studies of biological systems usually only allow qualitative information about topology and structure. However, in systems of high symmetry, exact analytical solutions containing information about specific distances can be obtained and used to fit high quality time-resolved data (usually within a global analysis methodology).
In this study, the tetrameric potassium channel KscA was engineered in order to have only a single tryptophan per monomer. An exact solution for homo-FRET within a square geometry was derived and fitted to anisotropy decays. The recovered distances will be compared with the ones from diffraction data, in both closed and open states, and for different ions. The advantage of this methodology over X-ray diffraction will be discussed.