Lecture: «Interaction of colloids with cells (in vitro and in vivo)»
Prof. Wolfgang Parak (Philipps Universität Marburg, Germany, and CIC biomaGUNE, San Sebastian, Spain)
CiQUS Seminar Room - 12.15 h.
Abstract
What happens to inorganic nanoparticles (NPs), such as plasmonic gold or silver, superparamagnetic iron oxide, or fluorescent quantum dot NPs, after they have been administrated to an animal or a human being? The review discusses the integrity, biodistribution, and fate of NPs after in vivo administration. First the hybrid nature of the NPs is described, by conceptually dividing them into the inorganic NP core, an engineered surface coating around the core which comprises the ligand shell and optionally also bioconjugation, and into the corona of adsorbed biological molecules. It is shown that in vivo all of these three compounds may degrade individually and that each of them can drastically modify the life-cycle and biodistribution of the whole hetero-structure. The NPs thus may be disintegrated into different parts, of which biodistribution and fate would need to be analyzed individually. Multiple labelling and quantification strategies for such purpose will be discussed. All reviewed data indicate that in vivo NPs no longer should be considered as homogeneous entity, but should be seen as inorganic/organic/biological nano-hybrids with complex and intricately linked degradation pathways.
About the speaker
Wolfgang J. Parak obtained his PhD at the LMU München, Germany (1999) in the group of Prof. Dr Hermann Gaub. After a postdoctoral stay at the University of California, Berkeley, CA, USA in the group of Prof. Dr Paul Alivisatos, he returned to Munich as an Assistant Professor in 2002 and was Associate Professor in 2005. Since 2007, he has been a Full Professor at the Physics Department of the Philipps Universität Marburg, Marburg, Germany. In addition, he was appointed as Head of the Biofunctional Nanomaterials Unit at CIC biomaGUNE, San Sebastian, Spain in 2013. Since 2010 he is also Associate Editor of ACS Nano. In 2009 Wolfgang J Parak won the "Nanoscience" - award 2008 from the Association of Nanotechnology-Centres Germany (AGenNT) and in 2012 he was awarded a Chinese Academy of Sciences Visiting Professorship for Senior International Scientists.