ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Student's work ECTS: 99 Hours of tutorials: 3 Expository Class: 24 Interactive Classroom: 24 Total: 150
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Physiology
Areas: Physiology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
- Identify the main existing techniques for genetic modification and their practical applications.
- Distinguish existing approaches for animal phenotyping.
- List the ethical principles and current legislation in relation to genetic manipulation and animal experimentation.
- Understand the importance of using genetically modified animal models to respond to a specific biomedical and/or biotechnological problem.
- Introduction to the use of genetically modified animals (GMA). GMA types.
- Specific ethical aspects of the use of GMA.
- Genetically modified mice (GMM). Transgenic mice. Generation of transgenic mice by DNA microinjection into fertilized oocyte nuclei. Other methods of generating transgenic mice (viral infection, manipulation of ES cells). Knock-out mice. Generation of knock-out mice by aggregation or microinjection of ES cells. Conditional models. Other types of GMM. Knock-in mice. Use of RNAi technology in the generation of GMM: knock-down mice. Genomic building techniques.
- Isolation of the gene of interest Use of the available bioinformatic resources necessary for the study prior to the design of the vector used to generate the GMM. Obtaining and subcloning the sequence of interest. Vector construction. Design and generation of the necessary vectors to obtain both transgenic animals and knock-out, knock-in and conditional models. GMM generation. High-quality DNA production that allows its subsequent microinjection into pronuclei of fertilized oocytes and for genetic modification through homologous recombination of ES cells. Genotyping of animals. Genotyped by DNA analysis: Southern Blot or PCR.
- Maintenance of GMM colonies. Facilities, equipment, care and management. Specific requirements of the sanitary state. Impact of the use of GMMs on the experimental design.
- Phenotypic characterization of animal models. Approaches to phenotypic analysis. Prenatal and postnatal effects. Influence of the genetic background on the phenotype. Techniques and examples of systems phenotyping.
Seminars
- Resolution of problems and questions.
Basic references:
Kaufman,M.H. & Bard,J.B.L. The Anatomical Basic of Mouse Development: Academic Press; 1999.
Papaioannou VE, Behringer RR. Mouse Phenotypes. A handbook of mutation analysis: CSHL Press; 2005.
Popesko P, Rajtova V, Horák J. A colour Atlas of anatomia of small laboratory animals. Vol. 2: Ed. Saunders; 2002.
Holland, EC. Mouse Models of Human Cancer. 1ª Ed: Wiley-VCH Verlag GmbH & Co. KGaA; 2004.
Behringer R, Gertsenstein M , Nagy K, Nagy A. Manipulating the Mouse Embryo: A Laboratory Manual, 4ª ed: CSHL Press; 2014.
Complementary references:
Nature Biotechnology
Trends in Biotechnology
Current Opinion in Biotechnology
Transgenic Research
Animal Reproduction Science
Links:
http://www.ncbi.nlm.nih.gov/pubmed/
http://emice.nci.nih.gov/emice/
http://www.eumorphia.org
http://www.bugalicia.org
http://busc.usc.es
https://secal.es/wp-content/uploads/2014/10/00-GENETICA-indice.pdf.pdf
Alternative support material:
The support material provided by the professors will be avaliable at CampusVirtual platform:
http://www.usc.es/campusvirtual/
Basic and general competences:
- Know the most important concepts, methods and results of the different branches of Biotechnology.
- Apply the theoretical-practical knowledge acquired in problem solving and the search for solutions in both academic and professional contexts.
- Know how to obtain and interpret relevant information and results and draw conclusions on issues related to Biotechnology.
- Be able to transmit information both in writing and orally and to discuss ideas, problems and solutions related to Biotechnology, before a general or specialized public.
- Study and learn autonomously, with organization of time and resources, new knowledge and techniques in Biotechnology and acquire the ability to work as a team.
- That the students have demonstrated to possess and understand the knowledge in an area of study that starts from the base of the general secondary education, and is usually found at a level that, although supported by advanced textbooks, also includes some aspects that involve knowledge from the forefront of their field of study.
- That students know how to apply their knowledge to their work or vocation in a professional way and possess the competencies that are usually demonstrated through the elaboration and defense of arguments and the resolution of problems within their area of study.
- That students have the ability to collect and interpret relevant data (usually within their study area) to make judgments that include a reflection on relevant social, scientific or ethical issues.
- That students can transmit information, ideas, problems and solutions to both a specialized and non-specialized audience.
- That the students have developed those learning skills necessary to undertake further studies with a high degree of autonomy
Tranversal competences
- Think in an integrated way and approach problems from different perspectives.
- Search, process, analyze and synthesize information from various sources.
- Organize and plan your work.
- Interpret experimental results and identify consistent and inconsistent elements.
- Maintain an ethical commitment.
- Teamwork.
- Critical reasoning
- Adaptation to new situations (resilience).
- Initiative and entrepreneurial spirit.
Specific competencies:
- Know and know how to apply instrumental techniques and work protocols in a laboratory, applying the regulations and techniques related to safety and hygiene, waste management and quality.
- Know the molecular bases and techniques for manipulating gene information in microorganisms, animals and plants and know how to properly apply these techniques in the different biotechnological fields.
Master classes, in which the teacher will explain the concepts of the subject with the support of audiovisual and computer media; It can have different formats (theory, problems and / or general examples, general guidelines for the subject), promoting student participation.
Seminars in small groups, in which applications of the theory are proposed and solved, exercises, problems, case simulations, etc. are carried out. Active participation of students is required.
Tutorials (individual or group) to clarify doubts, provide information or guide students, as well as to know the progress in the acquisition of skills.
In the Teaching Methodology, both synchronous and asynchronous telematic means will be used in order to contemplate the possibility of implementing the three possible scenarios: adapted normality, distancing and closure of the facilities.
Continuous assessment: The continuous assessment will be made through oral and/or written tests, assignments submitted and/or presented, student participation in the classroom and tutorials. (Weight 50%)
Final Exam: will include all the contents taught in the subject (Weight 50%)
In the Assessment System, both synchronous and asynchronous telematic means will be used in order to contemplate the possibility of implementing the three possible scenarios: adapted normality, distancing and closure of the facilities.
For cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of students' academic performance and the review of qualifications will apply.
Lectures: Hours 27 / Attendance 100%
Interactive class seminar: 21 hours / Attendance 100%
Group tutoring: 2 hours / Attendance 100%
Individualized tutorials: 1 hour / 100% attendance
Exam and review: 3 hours / Attendance 100%
Personal work: 96 hours / Attendance 0%
Previous knowledge in genetics, cellular, animal and molecular biology.
The three official languages of the course are Galician, Spanish and English. The teacher will decide which he/she will use depending on circumstances.
Contingency plan:
Both in the Teaching Methodology and in the Assessment System, both synchronous and asynchronous telematic means will be used in order to consider the possibility of implementing the three possible scenarios: adapted normality, distancing and closure of facilities.
Clara Alvarez Villamarin
- Department
- Physiology
- Area
- Physiology
- Phone
- 881815452
- clara.alvarez [at] usc.es
- Category
- Professor: University Professor
Victor Manuel Arce Vazquez
- Department
- Physiology
- Area
- Physiology
- Phone
- 881812291
- victor.arce [at] usc.es
- Category
- Professor: University Professor
Jose Antonio Costoya Puente
Coordinador/a- Department
- Physiology
- Area
- Physiology
- Phone
- 881815449
- josea.costoya [at] usc.es
- Category
- Professor: University Lecturer
Anxo Vidal Figueroa
- Department
- Physiology
- Area
- Physiology
- Phone
- 881815417
- anxo.vidal [at] usc.es
- Category
- Professor: Temporary PhD professor
Miguel Antonio Lopez Perez
- Department
- Physiology
- Area
- Physiology
- Phone
- 881815420
- m.lopez [at] usc.es
- Category
- Professor: University Lecturer
| Monday | |||
|---|---|---|---|
| 13:00-14:00 | Grupo /CLE_01 | Galician, Spanish | Virtual classroom |
| Tuesday | |||
| 13:00-14:00 | Grupo /CLE_01 | Spanish, Galician | Virtual classroom |
| 05.19.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 05.19.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
| 07.09.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 07.09.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |