ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Student's work ECTS: 99 Hours of tutorials: 3 Expository Class: 24 Interactive Classroom: 24 Total: 150
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology
Areas: Biochemistry and Molecular Biology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
- To identify the methods and applications of recombinant DNA technology.
- To identify the most used techniques and strategies for the production of recombinant proteins.
- To number gene-editing and genome sequencing technologies.
- To identify and apply experimental protocols for the management and analysis of nucleic acids.
-To know how to clone and express recombinant proteins.
Lectures and interactive seminar classes (27 h + 9 h)
BLOCK I. ESSENTIALS TOOLS AND BASIC TECHNIQUES (9 h)
Unit 1. Introduction to Genetic engineering (1 h)
Unit 2. Enzymology of recombinant DNA (3 h)
Unit 3. Basic techniques for isolation, purification and analysis of nucleic acids (3 h)
Unit 4. PCR (2 h)
BLOCK II. MOLECULAR CLONING (9 h)
Unit 5. Molecular cloning in bacterial cells (3.5 h)
Unit 6. Libraries (2 h)
Unit 7. Molecular cloning in eukaryotic cells (3.5 h)
BLOCK III. DNA MODIFICATION AND EXPRESSION OF RECOMBINANT DNA (9 h)
Unit 8. Site-directed mutagenesis. Introduction to protein engineering (3 h)
Unit 9. Gene inactivation and gene editing (1 h)
Unit 10. Expression of recombinant DNA in bacterial cells (3 h)
Unit 11. Expression of recombinant DNA in eukaryotic cells (2 h)
Seminars
-Resolution of problems and / or questions related to the contents of the program. (5 h)
-Management of databases and computer programs. (2 h)
-Preparation and presentation of a work from specific publications in the field of the subject. (2 h)
Laboratory (12 h)
Each student will have four work sessions in which they will perform:
-Sesions 1 and 2: Nucleic acid purification techniques. Transformation of bacterial cells with recombinant plasmids. PCR.
-Sesions 3 and 4: Expression and purification of recombinant proteins.
Small group tutoring (2 sessions of 1 hour) and individualized tutoring (1 hour): the first tutorial will be fundamentally for the presentation of the course and explanation of the work they have to do; the other two to clarify concepts or doubts about expository classes, seminars or practices.
Basic bibliography
-Brown, T.A., 2016. Gene cloning and DNA analysis: An Introduction. 7th ed. Chichester: Wiley-Blackwell.
-Clark, D.P. and Pazdernik, N.J., 2016. Biotechnology. [en liña] 2nd ed. Amsterdam: Elsevier Academic Press. Dispoñible en: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
-Perera, J., Tormo, A. y García, J.L., 2002. Ingeniería genética. 2 v. Madrid: Síntesis.
-Real García, M.D., Rausell Segarra, C. y Latorre Castillo, A., 2017. Técnicas de ingeniería genética. Madrid: Síntesis.
Complementary bibliography
-Clark, D.P, 2019. Molecular Biology. [en liña] 3rd ed. Amsterdam: Elsevier . Dispoñible en: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128132883/molecul…
-Glick, B.R. and Patten, C.L., 2017. Molecular biotechnology: principles and applications of recombinant DNA. 5th ed. Washington DC: ASM Press.
-Green, M.R. and Sambrook, J., 2012. Molecular cloning: a laboratory manual. 4th ed. 3 v. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
-McPherson, M.J. and Møller, S., 2006. PCR. 2nd ed. New York: Taylor & Francis.
-Renneberg, R., Berkling, V., and Loroch, V., 2016. Biotechnology for beginners [en liña]. Amsterdam: Elsevier. Dispoñible en: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128012246/biotech…
Others resources
- http://biomodel.uah.es. Web site of the Universidad Alcalá de Henares
-https://www.jove.com/science-education-library. Educational videos educativos about different molecular biology and genetic engineering techniques.
- http://www.ncbi.nlm.nih.gov/guide/. The National Center for Biotechnology Information, (NCBI) main source of data Banks (nucleotides, genes and genomes) and bioinformatics applications.
-Electronic resources from BUSC: Cell, Nature, Science, Sciencedirect, etc.
In scenario 3 the recommended bibliography is open access books marked with *, as well as the materials provided by the teacher and those mentioned under the heading "Other resources".
BASIC AND GENERAL COMPETENCES (CB and CG)
CB1 – Students must have shown to own knowledge and understanding in this study field which, starting from secondary education knowledge, manage advanced books and in some cases includes some issues which involve knowledge coming from the cutting edge of this study field.
CB2 – Students should know how to apply their knowledge to their job in a professional way and possess the competence and skills to argue and solve problems within their field.
CB3 – Ability to collect and interpret outstanding data (normally within their field of study) and be able to make judgments about relevant social, scientific or ethical events.
CB4 - Ability to communicate information, ideas, problems and solutions to a general or specialised audience.
CB5 – Students must have developed those learning skills needed to undertake post graduate studies with a high level of autonomy.
CG1 – To know the more outstanding concepts, methods and results from the different branches of Biotechnology.
CG2 – To apply the theoretical and practical knowledge acquired to solve problems in an academic and professional context.
CG3 – To know how to obtain and interpret relevant information and results and get conclusions about issues related to Biotechnology.
CG4 – Ability to communicate to a general or specialised audience both spoken and written information and to debate ideas, problems and solutions related to Biotechnology.
CG5 – Self-learning of new biotechnological knowledge and techniques, with an autonomous organization of time and resources, and acquire ability to work in group.
TRANSVERSAL SKILLS
CT1 – Students should think in an integrated way and approach problems from different perspectives.
CT2 – To search, manage, analyse and synthesise information from different sources.
CT4 – Interpret experimental results and identify consistent and inconsistent elements.
CT5 – To work in group.
CT6 – Critical reasoning.
CT7 – Students must maintain an ethical commitment.
SPECIFIC SKILLS
CE11– To know the molecular basis and techniques for manipulating gene information in microorganisms, animals and plants and to know how to properly apply these techniques in different biotechnological fields.
Scenario 1. Adapted normality
The general methodologies of the degree described in section 5.3 of the report will be followed. Thus, the following methodologies will be used:
- MASTER LECTURES face-to-face with computer projections and course in the on-line Campus (including material related to the classes and questionnaires).
- SEMINARS face-to-face in small groups, where problems or issues will be solved. The students will also be able to prepare and discuss topics related to the subject.
- TUTORIALS in very small groups for the clarification of doubts about the theory or practices, to provide information or guide the students. The first one will be face-to-face, the others will be held electronically.
- LABORATORY PRACTICAL CLASSES, in which the students, following the protocols prepared for this purpose, will handle the appropriate equipment and solve practical questions. Each student will have two work sessions in the laboratory; the rest of the hours will be completed with explanatory videos of analysis techniques, and interpretation of the results of the experiments.
Scenario 2. Distancing
- MASTER LECTURES: Depending on the situation and the availability of infrastructure, the exhibition classes may be carried out partially (shifts) or fully telematically (synchronous or asynchronous), preferably using the Teams platform..
- SEMINARS face-to-face in small groups, in alternate shifts.
- TUTORIALS in very small groups, to be held electronically.
- LABORATORY PRACTICAL CLASSES, will be the same as in scenario 1.
Scenario 3. Closure of facilities.
All teaching activities (master classes, seminars, tutorials and practical classes) will be held by distance learning, using the on-line Campus and Microsoft Teams platform.
- MASTER LECTURES will be asynchronous. Students will have the presentations of the classes recorded in the virtual classroom.
- SEMINARS will be synchronous, in small groups. Students will solve problems or issues raised in class or from the on-line Campus. They also will present a group work concerning a specific topic, via Teams.
- TUTORIALS in very small groups, synchronous.
- LABORATORY PRACTICAL CLASSES, work hours in the laboratory will be substituted by synchronous distance learning. Through videos and teacher explanations, students will learn how the practice protocol is developed, the basis of the techniques used and how the experimental results obtained are interpreted.
The distribution by students of teaching material (both written and audiovisual) to people outside the course is expressly prohibited.
1. The evaluation will consist of two parts:
1.1) Continuous evaluation (40% of the final grade), which in turn consists of:
i. Seminars (30% of the final grade). NON-MANDATORY ACTIVITY THAT CAN BE EVALUATED.
The evaluation will be based on the exercises / reports presented to the teacher or carried out in the seminars, and to the active participation in the academic activities. The lack of attendance at a minimum of 80% of the seminar hours implies the waiver of the right to seminars evaluation.
ii. Laboratory practices (10% of the final grade). EVALUABLE MANDATORY ACTIVITY.
For the grade, a final report and/or questions will be scored. Unexcused absences will imply a qualification of NOT SUITABLE. Students must obtain a qualification higher than 4 in the practices to overcome the subject.
The control of attendance to face-to-face activities will be by signature. Participation in telematic activities will be automatically registered in the on-line Campus and Microsoft Teams.
1.2) Final Test (60% of the final grade). EVALUABLE MANDATORY ACTIVITY, COMPLEMENTARY TO THE CONTINUOUS EVALUATION.
It will consist of questions / exercises related to the theoretical and practical content of the subject.
In scenario 1 it will be face-to-face, in scenarios 2 and 3 it will be synchronous telematics.
The qualification of the student will be obtained by weighing the final test with the continuous assessment (it is necessary to have a minimum score of 4 out of 10 points in the final exam to do the weighing).
2. Students who fail the course in the ordinary exam opportunity may retake the final test during the recovery opportunity (2nd opportunity). Scores obtained for the continuous evaluation will be maintained for the recovery opportunity. Those students who suspend the practices must do a recovery test.
3. Students who do not pass the subject but who have passed the laboratory practices will maintain those marks for the next academic year. Students who are enrolled in the class for the second time or more will be evaluated following the same guidelines for attendance to classes and calculating the final grade as students enrolled in the class for the first time.
In the final exam the competences will be evaluated: CB1/2/5, CG1/4/5, CT2/6, CE11.
In the seminars: CB1/2/3/4, CG1/3/4/5, CT1/2/5/6, CE11.
In the practices: CB2/4/5, CG1/3, CT4/5/7, CE11.
In cases of fraudulent carrying out of exercises or tests, the provisions of the "Student Assessment and Academic Performance Assessment Regulations" of the USC will apply.
In general, an average of 150 hours of work is estimated for this subject, of which 51 hours correspond to attendance at theoretical and practical classes, seminars and tutorials, 96 hours to self-employment of the student and the remaining 3 to the performance of exams and review of them.
- Assistance and participation in the proposed activities, as well as bringing the matter up to date.
-Consult of the recommended bibliography.
-Use of the virtual classroom.
-To make use of the tutorials for any type of matter related to the subject.
Contingency plan
Adaptations for scenarios 2 and 3 are as follows:
Teaching methodology
Scenario 2. Distancing
- MASTER LECTURES: Depending on the situation and the availability of infrastructure, the exhibition classes may be carried out partially (shifts) or fully telematically (synchronous or asynchronous), preferably using the Teams platform..
- SEMINARS face-to-face in small groups, where problems or issues will be solved. The students will also be able to prepare and discuss topics related to the subject.
- TUTORIALS in very small groups, will be by electronic means and synchronously.
- LABORATORY PRACTICAL CLASSES, will be the same as in scenario 1.
Scenario 3. Closure of facilities.
All teaching activities (master classes, seminars, tutorials and practical classes) will be held by distance learning, using the on-line Campus and Microsoft Teams platform.
- MASTER LECTURES will be asynchronous. Students will have the presentations of the classes recorded in the virtual classroom.
- SEMINARS will be synchronous. Students will solve problems or issues raised in class or from the on-line Campus. They also will present a group work concerning a specific topic, via Teams.
- TUTORIALS in very small groups, synchronously.
- LABORATORY PRACTICAL CLASSES, work hours in the laboratory will be substituted by synchronous distance learning. Through videos and teacher explanations, students will learn how the practice protocol is developed, the basis of the techniques used and how the experimental results obtained are interpreted.
Assessment system
The same evaluation system will be maintained for all three scenarios, except that all tests in Scenario 3 (and the final test in Scenario 2) will be conducted electronically.
Cristina Diaz Jullien
Coordinador/a- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816932
- cristina.diaz [at] usc.es
- Category
- Professor: Temporary PhD professor
Monday | |||
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16:00-17:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
Wednesday | |||
17:00-18:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
Thursday | |||
18:00-19:00 | Grupo /TI-ECTS03 | Spanish | Classroom 01. Charles Darwin |
18:00-19:00 | Grupo /TI-ECTS04 | Spanish | Classroom 01. Charles Darwin |
Friday | |||
18:00-19:00 | Grupo /TI-ECTS02 | Spanish | Classroom 01. Charles Darwin |
18:00-19:00 | Grupo /TI-ECTS01 | Spanish | Classroom 01. Charles Darwin |
05.28.2021 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
05.28.2021 10:00-14:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
07.15.2021 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |