ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.25 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.5
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology, Microbiology and Parasitology
Areas: Biochemistry and Molecular Biology, Microbiology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
The fundamental objectives of this subject are that students should know
1) the methods to obtain vaccines through the use of biotechnological tools.
2) the fundamentals and methods for the use of viruses for preventive and therapeutic purposes.
Theory programme (20h)
T1. Viral vectors in gene therapy. Design, production and application 2 h
T2. Viral vectors for the expression of recombinant proteins. Types and applications 2h.
T4 Viruses and nanotechnology. Virus like particles (VLPs), viral nanoparticles (VNP), nanoconjugates. Methods of obtaining and applications 2h.
T5. Oncolytic viruses. Viral types and applications 2h
T6. Biotechnological applications of bacteriophages 1h.
T7. Baculoviruses as biopesticides. Formulation and applications 1h
T 8. Vaccines: definition, characteristics and composition. History of vaccination. 2h
T9. Classification of vaccines. Characteristics, benefits, problems of different types of vaccines. 2h
T10. Vaccination guidelines and routes of administration. Adjuvants. Definition and mechanisms of action. Traditionally used compounds and new research. 1h
T 11. Design. Identification of response-inducing antigens. New generation vaccines. 2h
T 12. Development of procedures for industrial vaccine production. Control of vaccines: legal and sanitary aspects. Requirements for vaccine registration. 1h
T 13. Vaccination: current challenges and strategies. 2h
Seminar programme (5 h)
Development of practical exercises and problem solving related to theory topics. (activity carried out online)
Presentation of work performed by the students and discussion on the exercises carried out and specific aspects of the subject.
Practical programme (12 h)
Session 1: Genetic manipulation of viruses. 2h.
Session 2: Manipulation of cell cultures and transfection 2h
Session 3: Virus culture and recombinant protein detection: 2h
Session 4: Basis and expression of a subunit vaccine in bacteria 3h
Session 5: Purification and characterisation of subunit vaccine 3h
Translated with www.DeepL.com/Translator (free version)
basic biblography
Bloom, B.R. and Lambert, P.H. (eds.) 2016. The vaccine book. Amsterdam: Elsevier
Flint, J., Racaniello, V.R., Rall, G. F., Hatziioannou, T., Skalka, A.M. 2020. Principles of virology. Hoboken: Wiley
Khan, M.S., Kahn I.A., Barh D. (eds.) 2019. Applied molecular biotechnology. The Next generation of genetic engineering. Boca Raton: CRC Press
Tennat P., Fermín G., Foster J.E. 2018. Viruses: molecular biology, host interactions, and applications to biotechnology. London: Academic Press
Complementary bibliography
Cann, A.J. 2015. Principles of molecular virology. 6th ed. Amsterdam: Academic Press
BASICS
Those specified in the degree report.
GENERAL
Those specified in the degree report.
TRANSVERSAL
TC1 - Think in an integrated way and approach problems from different perspectives.
TC2 - Search, process, analyse and synthesise information from different sources.
TC3 - Organise and plan their work.
TC4 - Interpret experimental results and identify consistent and inconsistent elements.
TC5 - Work in a team.
TC6- Reason critically.
SPECIFIC
SC1 - Know how to make calculations, analyse data and interpret experimental results specific to the fields of Biotechnology.
SC3 - Knowing and knowing how to apply instrumental techniques and work protocols in a laboratory, applying regulations and techniques related to health and safety, waste management and quality.
SC5 - Knowing how to obtain and interpret information from the main databases (biological, omics, bibliographic) and use bioinformatics tools.
SC9 - Knowing how to identify the different types of viruses, microorganisms and animal and plant tissues, understanding their development, organisation and physiology, and knowing their application in the field of Biotechnology.
SC11 - Knowing the molecular bases and techniques for manipulating gene information in microorganisms, animals and plants and knowing how to apply these techniques appropriately in different biotechnological fields.
The teaching methodology will be based on the lecture, providing concepts, diagrams and photographs through audiovisual media and will encourage active student participation through the formulation of questions or the posing of practical cases to be solved in the classroom. The development of practical exercises and problem solving will be promoted (online activity), which will be corrected during the face-to-face seminars, in which the work carried out by the students will also be presented (activity aimed at promoting the use of bibliography, interpretation and synthesis).
Practical classes will be held in the laboratory.
1-hour tutorials will be scheduled for the resolution of doubts.
Cvaluation
1. Compulsory activities
1.1 Demonstration of the acquisition of the concepts of the subject.
Theoretical examination* The student's theoretical knowledge will be assessed by definition of basic concepts, answers to short questions, test questions and interpretation of images and diagrams. In addition to the contents, clarity and conciseness will be taken into account: 65% (maximum mark 6.5).
CG3, GG4, CT1, CT2, CE1, CE9, CE11].
1.2. Completion of the internship**.
- Evaluation of the internship report (its presentation, the quality of the report and the description and explanation of the tests carried out will be taken into account): 10% (maximum mark 1)
CT4, CE1, CE3] [CT4, CE1, CE3 are assessed].
2. Continuous assessment (optional activities)
Completion of practical work or case studies/problems**: 25%.
- Presentation and report of seminar work (the quality of the report and the presentation will be taken into account, as well as the confidence in answering the questions posed): 15% (maximum mark 1.5)
- Resolution of exercises, problems and case studies (their presentation and the reasoning behind their resolution will be taken into account): 10% (maximum mark 1).
CB3, CB4, CG3, CG4, CT1, CT2, CT3, CT5, CT6, CE5, CE9, CE11].
* The theory exam will be taken in writing, according to the date established by the dean's team, as published in the student guide of this Faculty. Optionally, students who so wish may take the oral exam, on a date previously agreed with the interested party(s). The mark of the theory exam can only be maintained until the 2nd opportunity exam of the current academic year. It will be necessary to pass the theory exam (5) to add the rest of the items.
** The mark for the practicals and the continuous assessment activities will be maintained for two academic years if the student so requests.
Total presential hours 40,5
Total non presential hours 72
Knowledge of biochemistry, genetics and microbiology is required to take the course.
The subject will have a virtual classroom on the Moodle platform in which, in addition to the contents developed by the teachers, other useful information will be available to students
For cases of fraudulent performance of exercises or tests, the provisions of the "Normativa de avaliación do rendemento académico dos estudantes e de revisión de cualificacións" (Regulations for the assessment of students' academic performance and review of qualifications) shall apply.
Jose Manuel Martinez Costas
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881815734
- jose.martinez.costas [at] usc.es
- Category
- Professor: University Professor
Sandra Souto Pereira
- Department
- Microbiology and Parasitology
- Area
- Microbiology
- sandra.souto [at] usc.es
- Category
- Xunta Post-doctoral Contract
| Monday | |||
|---|---|---|---|
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 09. Barbara McClintock |
| Tuesday | |||
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 09. Barbara McClintock |
| 05.22.2023 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 07.05.2023 10:00-14:00 | Grupo /CLE_01 | Classroom 08. Louis Pasteur |