ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Student's work ECTS: 51 Hours of tutorials: 3 Expository Class: 9 Interactive Classroom: 12 Total: 75
Use languages Spanish, Galician, English
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Physical Chemistry
Areas: Physical Chemistry
Center Faculty of Chemistry
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable | 1st year (Yes)
The general aim of this course is that the students learn the fundamental aspects of electronic spectroscopy, in particular of fluorescence, and photochemistry. Special attention will be paid to the utility of fluorescence to know the molecular behavior in excited electronic states and in the applications of fluorescence in Chemistry, Biology and Medicine. At the end of the course the student should be able:
• To understand the fundamentals of electronic spectroscopy and fluorescence and the molecular features in excited electronic states.
• To know the fluorescence techniques to measure fluorescence.
• To describe the fluorescence quenching mechanisms and their utility.
• To understand the mechanisms of electronic energy transfer and their use in structural studies.
• To know how to use different fluorescence methods to obtain structural and dynamic information about the molecular and supramolecular environment.
• To know the most important types of fluorescence probes and their applications.
• To do fluorescence measurements confidently and correctly.
1. Fundamentals of electronic spectroscopy and fluorescence spectroscopy
Luminiscent phenomena. Radiative and nonradiative processes. Fluorescence excitation and emission spectra. Fluorescence quantum yield. Fluorescence lifetime. Effect of environment on fluorescence.
2. Experimental techniques
Measurement of fluorescence spectra: the spectrofluorometer. Correction of excitation and emission spectra. Measurement of fluorescence lifetimes. Measurement of fluorescence polarization. Ultrafast techniques. Single-molecule fluorescence. Fluorescence Microscopy.
3. Fluorescence quenching
Collisional or dynamic quenching. Stern-Volmer equation. Static quenching. Static and dynamic quenching. Applications to study complex formation and microheterogeneous systems.
4. Excited electronic states and photochemistry
Excited-state complex formation: excimers and exciplexes. Photoinduced electron transfer. Photoinduced proton transfer. Other photochemical reactions.
5. Electronic energy transfer
Electronic energy-transfer mechanisms. Förster Resonance Energy Transfer (FRET). Applications for the measurement of molecular distances and the study of supramolecular associations. Dexter mechanism of energy transfer: photosensitization and photodynamic therapy.
6. Fluorescence probes
Classes of fluorescence probes: intrinsic and extrinsic. Green Fluorescence Protein. Quantum dots. Applications in biomedicine, analyses, environment, and materials studies.
Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd Ed. Springer, New York, 2006.
Bernard Valeur, Molecular Fluorescence. Principles and Applications, 2nd Ed. Wiley-VCH, Weinheim, 2012.
Petr Klán and Jacob Wirz, Photochemistry of Organic Compounds: From Concepts to Practice, Wiley, Chichester, 2009.
Paul R. Selvin y Taekjip Ha, Single-Molecule Techniques. A laboratory manual, Cold Spring Harbor Laboratory Press, New York, 2008.
Review and research articles related to the subject.
Basic and general-subject skills:
CG2. To obtain information from the scientific literature using the appropriate channels and to use the above mentioned information to raise and contextualize a research topic.
CG5. To use scientific terminology in English to discuss experimental results in a professional chemical context.
CG6. To apply correctly the new information technologies to solve problems in the professional activity.
CB7. To apply the acquired knowledge and the ability to solve problems in new environments and in a multidisciplinary context related to the area of study.
CB8. To integrate knowledge and to make judgments from an information, taking into account the social responsibilities and ethics linked to the application of this knowledge and judgments.
Transversal skills:
CT1. To elaborate, write and defend in public scientific and technical reports.
CT3. To work with autonomy and efficiency in the daily practise of the research or the professional activity.
CT4. To appreciate the value of quality and continuous improvement, being rigorous and responsible and behaving with professional ethics.
Subject-specific skills:
CE1. To define specialized concepts, principles, theories and facts of the different Chemistry areas.
CE4. To innovate in the synthesis and chemical analysis methods related to the different areas in Chemistry.
CE7. To work with advanced instrumentation for chemical and structural analysis.
MD1. Lectures (using blackboard, computer, projector), complemented with virtual teaching.
MD3. Seminars conducted by lecturers from the Master or by invited professionals from industry, administration or other universities.
MD4. Resolution of practical exercises (problems, test questions, information interpretation and processing, referring scientific publications, etc.).
MD5. Individual tutorial classes or in small groups.
MD6. Individually or in-group written assignments, about scientific topics related to the different master subjects.
MD7. Oral presentations of reports including discussions with teachers and students.
MD8. Using specialized software and internet. Educational on-line support (Campus Virtual).
MD9. Experimental work using basic techniques in the laboratory.
MD10. Personal study based on the different information sources.
MD11. Exams to verify the students´ theoretical and practical knowledge, abilities, and attitudes.
CONTINGENCY PLAN FOR REMOTE TEACHING ACTIVITIES:
The remote teaching activities would be carried out synchronously/asynchronously and always according to the schedule established by the center, through the different telematic means available at the USC, preferably the Virtual Campus and MS Teams.
Seminars and tutorials, as well as the direct communication both between the students themselves and between them and the teacher, can be done through discussion forums in the Virtual Campus, through MS Teams or, in exceptional cases, by email.
Student assessment will be done by means of a summative assessment and a final exam. Attendance to at least 80 % of the obligatory interactive classes (seminars and tutorials) is necessary for a student to be allowed to take the final exam.
• Summative assessment (40%), consisting of the following contributions:
Problems and practical cases: 10%.
Laboratory: 20%.
Monographic work (report and oral presentation): 10%.
• Final exam (60%), including all the subject contents.
Students repeating the course will have to follow the same attendance and assessment rules as students doing the course for the first time.
PLAGIARISM AND MISUSE OF TECHNOLOGIES IN THE CONDUCT OF TASKS OR TESTS: "For cases of fraudulent execution of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and revision of qualifications will apply."
CONTINGENCY PLAN FOR REMOTE TEACHING ACTIVITIES: The evaluation system will be the same regardless of the type of teaching used (face-to-face or virtual), with the only difference that the evaluation activities will be carried out, according to what the competent authorities establish, either in person in the classroom or remotely through the telematic means available at the USC.
In-class work time:
Lectures: 12 h
Seminars: 7 h
Tutorials: 2 h
SUBTOTAL: 21 h
Out-of-class work time:
Assignments, solving exercises: 20 h
Study time: 34 h
TOTAL: 75 h
• Attendance to lectures is highly recommended.
• It is important to keep up to date in studying the course material.
• After reading a chapter in any of the recommended books, it is useful to write a summary of the important points.
• Working problems is essential to learning the course contents.
The classes are given in Spanish, Galician and/or English depending on the preferences of the students.
CONTINGENCY PLAN
METHODOLOGY
CONTINGENCY PLAN FOR REMOTE TEACHING ACTIVITIES:
The remote teaching activities would be carried out synchronously/asynchronously and always according to the schedule established by the center, through the different telematic means available at the USC, preferably the Virtual Campus and MS Teams.
Seminars and tutorials, as well as the direct communication both between the students themselves and between them and the teacher, can be done through discussion forums in the Virtual Campus, through MS Teams or, in exceptional cases, by email.
ASSESSMENT SYSTEM:
PLAGIARISM AND MISUSE OF TECHNOLOGIES IN THE CONDUCT OF TASKS OR TESTS: "For cases of fraudulent execution of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and revision of qualifications will apply."
CONTINGENCY PLAN FOR REMOTE TEACHING ACTIVITIES: The evaluation system will be the same regardless of the type of teaching used (face-to-face or virtual), with the only difference that the evaluation activities will be carried out, according to what the competent authorities establish, either in person in the classroom or remotely through the telematic means available at the USC.
Maria De La Merced Novo Rodriguez
Coordinador/a- Department
- Physical Chemistry
- Area
- Physical Chemistry
- m.novo [at] usc.es
- Category
- Professor: University Lecturer
Wajih Al-Soufi
- Department
- Physical Chemistry
- Area
- Physical Chemistry
- Phone
- 982824114
- wajih.al-soufi [at] usc.es
- Category
- Professor: University Lecturer
| Monday | |||
|---|---|---|---|
| 12:00-14:00 | Grupo /CLE_01 | Spanish | Classroom 2.12 |
| 01.18.2021 16:00-20:00 | Grupo /CLE_01 | Mathematics Classroom (3rd floor) |