ILS and Proteins (Enzymes)

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secundo
secundo's picture
ILS and Proteins (Enzymes)

Hi.

I am interested in the use of ILs as reaction media for biocatalytic reactions or as media for proteins for other biotechnological applications (e.g., biosensors).

Is there any of you interested in the same application?

A good starting point for discussion might be the following paper:

F. van Rantwijk, F. Secundo  and R. A. Sheldon. “Structure and activity of Candida antarctica lipase B in ionic liquids”  Green Chemistry, 8(3), 283–286, (2006).

I would be happy to start a discussion and possibly collaborations on this subject.

Francesco Secundo

maia_guncheva
maia_guncheva's picture
Hi Francesco,

Hi Francesco,

I am also interested in the same application of ILs. I have been studied stability, activity and specificity of some bacterial lipases in non-conventional medium.

Maya Guncheva

 

Doumeche
Doumeche's picture
ILS and Proteins (Enzymes)

Hello,

I'm actually new in this working group. I'm working with Ils and enzymes since a few years. I'll be glad to share my knowledge on the field with the group and discuss about collaboration on the field.

Sincerely Yours,

 

Bastien

secundo
secundo's picture
Let's discuss about ILs and proteins

Hi all.

According to your exeprience, what are the properties that an ionic liquid should have to stabilize a protein? Can you recomend articles that have investigated this aspect in detail?

Thanks

Francesco

bminoofar
bminoofar's picture
Hi All,

Hi All,

I am also interested in ILS and Proteins, Enzymes and DNA but from computational point of view. I will be gald if we can discuss more about it too.

Thanks 

Babak Minofar 

lsaraiva
lsaraiva's picture
ILs and enzymes

Hello,

I have been working mainly in the automation of biocatalytic assays in mixtures of ionic liquids and water, aiming the solubilization of less polar substrates, the immobilization of enzyme, its activation with ILs and surfactants and ILs, and its inhibition by ILs as  means of evauating its toxicity to the environment. 

The results appear and sometimes we don´t know exactly why. Computational studies between enzymes active site is I think a way of getting a clue about it.

So, I am open at discussion and colaboration

M. Lúcia MFS Saraiva