Publications

CRISPR/Cas9 Delivery Systems to Enhance Gene Editing Efficiency

Ana Seijas, Diego Cora, Mercedes Novo, Wajih Al-Soufi, Laura Sánchez, Álvaro J. Arana

Preprints. https://doi.org/10.20944/preprints202503.2038.v1

Amyloid Capture and Aggregation Inhibition by Human Serum Albumin

Diego Cora, Wajih Al‐Soufi, Mercedes Novo

International Journal of Biological Macromolecules, Volume 301, April 2025, 140367

https://doi.org/10.1016/j.ijbiomac.2025.140367

(Preprint: ChemRxiv, 2024, Version 1, 10.26434/chemrxiv-2024-fr8gt)

Thiazole Orange as fluorescent marker for the detection and quantification of dsDNA

Stella Hernández, Lucas Piñeiro, Wajih Al-Soufi, Mercedes Novo

ChemRxiv, 2024, Version 1, Preprint, 10.26434/chemrxiv-2024-lbxzx

Critical aggregation concentration and reversibility of amyloid-β (1–40) oligomers

Sara Illodo, Wajih Al-Soufi, Mercedes Novo

Archives of Biochemistry and Biophysics, 2024, 761, 110179, DOI 10.1016/j.abb.2024.110179

Early aggregation of Amyloid-β (1-42) studied by Fluorescence Correlation Spectroscopy

Mercedes Novo, Cibrán Pérez-González, Sonia Freire and Wajih Al-Soufi

Methods in Molecular Biology, 2023, 2551, 1–14. DOI: 10.1007/978-1-0716-2597-2_1

Supramolecular association studied by Fluorescence Correlation Spectroscopy

Mercedes Novo and Wajih Al-Soufi

Frontiers in Chemistry 2022, 10, 1042658. DOI: 10.3389/fchem.2022.1042658 (Open Access)

Spectroscopic Characterization of Mitochondrial G-Quadruplexes

Sara Illodo, Cibrán Pérez-González, Ramiro Barcia, Flor Rodríguez-Prieto, Wajih Al-Soufi, Mercedes Novo

International Journal of Molecular Sciences 2022, 23(2), 925. DOI: 10.3390/ijms23020925 (Open Access)

A Surfactant Concentration Model for the Systematic Determination of the Critical Micellar Concentration and the Transition Width (Review)

Wajih Al-Soufi and Mercedes Novo

Molecules 2021, 26, no. 17: 5339. https://doi.org/10.3390/molecules26175339 (Open Access)

When the Kitchen Turns into a Physical Chemistry Lab

Wajih Al-Soufi, Jorge Carrazana-Garcia, and Mercedes Novo

Journal of Chemical Education 2020, 97, 9, 3090–3096, https://doi.org/10.1021/acs.jchemed.0c00745

Unveiling the multi-step solubilization mechanism of sub-micron size vesicles by detergents

Paul A. Dalgarno, José Juan-Colás, Gordon J. Hedley, Lucas Piñeiro, Mercedes Novo, Cibrán Pérez-González, Ifor D. W. Samuel, Mark C. Leake, Steven Johnson, Wajih Al-Soufi, J. Carlos Penedo, Steven D. Quinn

Scientific Reports 2019, 9, Article number: 12897, DOI: 10.1038/s41598-019-49210-0 (Open Access)

Critical aggregation concentration for the formation of early Amyloid-β (1–42) oligomers

Mercedes Novo, Sonia Freire, Wajih Al-Soufi

Scientific Reports 2018, 8, Article number: 1783, DOI: 10.1038/s41598-018-19961-3 (Open Access)

Fluorescence-Labeled Bis-benzamidines as Fluorogenic DNA Minor-Groove Binders: Photophysics and Binding Dynamics

Jorge Bordello, Mateo I. Sánchez, M. Eugenio Vázquez, José L. Mascareñas, Wajih Al-Soufi, and Mercedes Novo

Chemistry - A European Journal 2015, 21, 1609–1619. DOI: 10.1002/chem.201404926

Accepted Author Manuscript

Towards ratiometric sensing of amyloid fibrils in vitro

Sonia Freire, Flor Rodríguez-Prieto, M. Carmen Ríos Rodríguez, J. Carlos Penedo, Wajih Al-Soufi, and Mercedes Novo

Chemistry - A European Journal 2015, 21, 3425–3434. DOI: 10.1002/chem.201406110

Accepted Author Manuscript

Fluorescence Emission of Pyrene in Surfactant Solutions

Lucas Piñeiro, Mercedes Novo, and Wajih Al-Soufi

Advances in Colloid and Interface Science 2015, 215, 1–12. DOI:10.1016/j.cis.2014.10.010

Author post-print and Supplementary Information

Quick adjustment of imaging tracer payload, for in vivo applications of theranostic nanostructures in the brain

Jesús Agulla, David Brea, Bárbara Argibay, Mercedes Novo, Francisco Campos, Tomás Sobrino, Miguel Blanco, José Castillo, Pedro Ramos-Cabrer

Nanomedicine : Nanotechnology, Biology, and Medicine 2014, 10 (4), 851–858, DOI: 10.1016/j.nano.2013.12.004)

Photophysical study of Thioflavin T as fluorescence marker of amyloid fibrils

Sonia Freire, Marcus H. de Araujo, Wajih Al-Soufi, Mercedes Novo

Dyes and Pigments 2014, 110, 97-105

DOI 10.1016/j.dyepig.2014.05.004 Accepted Author Manuscript (AAM)

In Vivo Theranostics at the Peri-Infarct Region in Cerebral Ischemia

Jesús Agulla, David Brea, Francisco Campos, Tomás Sobrino, Bárbara Argibay, Wajih Al-Soufi, Miguel Blanco, José Castillo, Pedro Ramos-Cabrer

Theranostics 2014, 4, 90-105, DOI:10.7150/thno.7088

Dye exchange in micellar solutions. Quantitative analysis of bulk and single molecule fluorescence titrations

Lucas Piñeiro, Sonia Freire, Jorge Bordello, Mercedes Novo, and Wajih Al-Soufi

Soft Matter 2013, 9, 10779-10790, DOI: 10.1039/ c3sm52092g (pdf)

Single-Molecule Approach to DNA Minor-Groove Association Dynamics

Jorge Bordello, Mateo I. Sánchez, M. Eugenio Vázquez, José L. Mascareñas, Wajih Al-Soufi and Mercedes Novo

Angewandte Chemie International Edition 2012, 51, 7541–7544

A Model for Monomer and Micellar Concentrations in Surfactant Solutions. Application to Conductivity, NMR, Diffusion and Surface Tension data

Wajih Al-Soufi, Lucas Piñeiro, Mercedes Novo

Journal of Colloid and Interface Science 2012, 370, 102–110 DOI: 10.1016/j.jcis.2011.12.037 (pdf) (Supplementary Information)

Principal component global analysis of series of fluorescence spectra

Wajih Al-Soufi, Mercedes Novo, Manuel Mosquera, and Flor Rodríguez-Prieto

in Reviews in Fluorescence, volume 2009, 2011, 6, 23–45, ed. Chris D. Geddes, Springer, New York

Host-guest association studied by fluorescence correlation spectroscopy.

Mercedes Novo, Daniel Granadero, Jorge Bordello and Wajih Al-Soufi

Journal of Inclusion Phenomena and Macrocyclic Chemistry 2011, 70, 259-268

Host-guest complexation studied by fluorescence correlation spectroscopy: Adamantane-cyclodextrin inclusion

Daniel Granadero, Jorge Bordello, María Jesús Pérez-Alvite, Mercedes Novo, and Wajih Al-Soufi

International Journal of Molecular Sciences 2010, 11, 173–188

Exchange-dynamics of a neutral hydrophobic dye in micellar solutions studied by Fluorescence Correlation Spectroscopy

Jorge Bordello, Mercedes Novo and Wajih Al-Soufi

Journal of Colloid and Interface Science 2010, 345, 369–376

Role of electrostatic and hydrophobic forces in the interaction of ionic dyes with charged micelles

Sonia Freire, Jorge Bordello, Daniel Granadero, Wajih Al Soufi and Mercedes Novo

Photochemical & Photobiological Sciences 2010, 9, 687–696

Host-assisted guest self-assembly: enhancement of the dimerization of pyronines Y and B by γ-cyclodextrin

J. Bordello, B. Reija, W. Al-Soufi, M. Novo

ChemPhysChem 2009, 10, 931–939

Dynamics of supramolecular association monitored by fluorescence correlation spectroscopy (Minireview)

W. Al-Soufi, B. Reija, S. Felekyan, C. A. Seidel, M. Novo

ChemPhysChem 2008, 9, 1819–1827

Dye-Exchange Dynamics in Micellar Solutions Studied by Fluorescence Correlation Spectroscopy

M. Novo, S. Felekyan, C. A. M. Seidel, and W. Al-Soufi

Journal of Physical Chemistry B 2007, 111, 3614-3624

Freezing point of milk: a natural way to understand colligative properties

Mercedes Novo, Belén Reija, Wajih Al-Soufi

Journal of Chemical Education 2007, 84, 1673-1675

Fluorescence correlation spectroscopy, a tool to investigate supramolecular dynamics: Inclusion complexes of pyronines with cyclodextrin

Wajih Al-Soufi, Belén Reija, Mercedes Novo, Suren Felekyan, Ralf Künemuth, and Claus A. M. Seidel

Journal of the American Chemical Society 2005, 127, 8775-8784

Specific interactions in the inclusion complexes of pyronines Y and B with beta-cyclodextrin

Belén Reija, Wajih Al-Soufi, Mercedes Novo, José Vázquez Tato

Journal of Physical Chemistry B 2005, 109, 1364-1370

Complexation of methyl orange with beta-cyclodextrin: detailed analysis and application to quantification of polymer-bound cyclodextrin

Jorge Carrazana, Belén Reija, Pedro Ramos Cabrer, Wajih Al-Soufi, Mercedes Novo, José Vázquez Tato

Supramolecular Chemistry 16, 549-559 (2004)

"Three-in-one" complexes formed by anionic guests and monosubstituted cationic alkyldiamino beta-cyclodextrin derivatives

Emilio Álvarez-Parrilla, Pedro Ramos Cabrer, Laura A. De La Rosa, Wajih Al-Soufi, F. Meijide, and José Vázquez Tato

Supramolecular Chemistry 15, 207-211 (2003)

Determination of second-order association constants by global analysis of 1H and 13C NMR chemical shifts. Application to the complexation of sodium fusidate and potassium helvolate by b- and g-cyclodextrin

Wajih Al-Soufi, Pedro Ramos Cabrer, Aida Jover, Rosane M. Budal, and José Vázquez Tato

Steroids 68, 43-53 (2003)

Complexation of bile salts by natural cyclodextrins

Pedro Ramos Cabrer, Emilio Álvarez-Parrilla, Wajih Al-Soufi, Francisco Meijide, Eugenio Rodríguez Núñez, and José Vázquez Tato

Supramolecular Chemistry 15, 33-43 (2003)

Complexation of Several Fungicides with β-Cyclodextrin: Determination of the Association Constants and Isolation of the Solid Complexes

Minerva Lezcano, Mercedes Novo, Wajih Al-Soufi, Eugenio Rodríguez-Núńez, and José Vázquez Tato

Journal of Agricultural and Food Chemistry 51, 5036-5040 (2003)

Spectra and structure of complexes formed by sodium fusidate and potassium helvolate with γ- and β-cyclodextrin

Aida Jover, Rosane M. Budal, Wajih Al-Soufi, Francisco Meijide, José Vázquez Tato, and Rosendo A Yunes

Steroids 68, 55-64 (2003)

2,2'-Bipyridyl-3,3'-diol Incorporated into AlPO4-5 Crystals and Its Spectroscopic Properties as Related to Aqueous Liquid Media

Knut Rurack, Katrin Hoffmann, Wajih Al-Soufi, and Ute Resch-Genger

Journal of Physical Chemistry B 106, 9744-9752 (2002)

Complexation of several benzimidazole-type fungicides with alpha- and beta-cyclodextrins

Minerva Lezcano, Wajih Al-Soufi, Mercedes Novo, Eugenio Rodríguez-Núńez, and José Vázquez Tato

Journal of Agricultural and Food Chemistry 50, 108-112 (2002)

Supramolecular linear conglomerates formed by β-cyclodextrin dimers and sodium deoxycholate

Emilio Álvarez-Parrilla, Pedro Ramos Cabrer, Anand Pal Singh, Wajih Al-Soufi, Francisco Meijide, Eugenio Rodríguez Núńez, and José Vázquez Tato

Supramolecular Chemistry 14, 397-404 (2002)

Principal component global analysis of fluorescence and absorption spectra of 2-(2'-hydroxyphenyl)benzimidazole

W. Al-Soufi, M. Novo, and M. Mosquera

Applied Spectroscopy 2001, 55, 630–636

Resolution of the Association Equilibria of 2-(p-Toluidinyl)-naphthalene-6-sulfonate (TNS) with β-Cyclodextrin and a Charged Derivative

Emilio Álvarez-Parrilla, Wajih Al-Soufi, Pedro Ramos Cabrer, Mercedes Novo, and José Vázquez Tato

Journal of Physical Chemistry B 105, 5994-6003 (2001)

Dendritic growth of a supramolecular complex

Emilio Álvarez-Parrilla, Pedro Ramos Cabrer, Wajih Al-Soufi, Francisco Meijide, Eugenio Rodríguez Núńez, and José Vázquez Tato

Angewandte Chemie, International Edition in English 39, 2856-2858 (2000)

Influence of pH on the stability of the inclusion complex of methyl orange and beta-cyclodextrin

Carrazana García, J., Al-Soufi, W., Novo Rodríguez, M., and Vázquez Tato, J.

Proceedings of the 9th International Symposium on Cyclodextrins, 1998, Santiago de Compostela, Spain.
Kluwer Academic Publishers, Dordrecht, 179-182 (1999)

Chemical instability of 5-aminolevulinic acid used in the fluorescence diagnosis of bladder tumors

Mercedes Novo, Gereon Huettmann, and Heyke Diddens

Journal of Photochemistry and Photobiology, B: Biology 34, 143-148 (1996)

Excited-state behavior of 2-(4’-pyridyl)benzimidazole in aqueous solution: Proton-transfer processes and dual fluorescence

M. Novo, M. Mosquera, and F. Rodríguez Prieto

Journal of Physical Chemistry 1995, 99, 14726–14732

Influence of acidity on the fluorescence spectra of 2-pyridyl-benzimidazoles in aqueous solution

Mercedes Novo, Manuel Mosquera, and Flor Rodríguez Prieto

Journal of the Chemical Society, Faraday Transactions 1993, 89, 885–889

Photoinduced electron transfer in and electron injection from trichromophoric molecules forming Langmuir Blodgett films

Danny Meerschaut, Mercedes Novo, Mark Van der Auweraer, Wim Dehaen, and Frans C de Schryver

Molecular Crystals and Liquid Crystals Science and Technology, Section A: Molecular Crystals and Liquid Crystals 235, 67-74 (1993)

Anomalous field dependence of the hole mobility in a molecular doped polymer

M. Novo, M. van der Auweraer, F. C. de Schryver, P. Borsenberger, and H. Baessler

Physica Status Solidi B: Basic Research 177, 223-41 (1993)

Tautomerism - to be taken lightly

Flor Rodríguez Prieto, Mercedes Novo, José A. Domínguez Herbón, and Berta Fernández Rodríguez

Education in Chemistry 1992, 29, 139–141

Prototropic equilibria of 2-pyridylbenzimidazoles in aqueous solution

Mercedes Novo, Manuel Mosquera, and Flor Rodríguez Prieto

Canadian Journal of Chemistry 1992, 70, 823–827

Keto-enol tautomerization of 2-(2-hydroxyphenyl)benzoxazole and 2-(2-hydroxy-4-methylphenyl)benzoxazole in the triplet state: hydrogen tunneling and isotope effects. 2. Dual phosphorescence kinetics

Heike Eisenberger, Bernhard Nickel, A. Andreas Ruth, Wajih Al-Soufi, Karl H. Grellmann, and Mercedes Novo

Journal of Physical Chemistry 95, 10509-10518 (1991)

Kinetics of photoenolization of 5,8-dimethyl-1-tetralone: hydrogen-transfer tunnel-effects in the excited triplet state

W. Al-Soufi, A. Eychmueller, and K. H Grellmann

Journal of Physical Chemistry 95, 2022-2026 (1991)

Keto-enol tautomerization of 2-(2'-hydroxyphenyl)benzoxazole and 2-(2'-hydroxy-4'-methylphenyl)benzoxazole in the triplet state: hydrogen tunneling and isotope effects. 1. Transient absorption kinetics

Wajih Al-Soufi, Karl H. Grellmann, and Bernhard Nickel

Journal of Physical Chemistry 95, 10503-10509 (1991)

Dual fluorescence of 2-(2’-pyridyl)benzimidazole in aqueous solution due to photoinduced proton-transfer processes

Flor Rodríguez Prieto, Manuel Mosquera, and Mercedes Novo

Journal of Physical Chemistry 1990, 94, 8536–8542

Triplet state formation and cis->trans isomerization in the excited singlet state of the keto tautomer of 2-(2'-hydroxyphenyl)benzothiazole

Wajih Al-Soufi, Karl H. Grellmann, and Bernhard Nickel

Chemical Physics Letters 174, 609-16 (1990)

Mechanistic studies of organic photoreactions utilizing proton tunnel effects

Karl Heinz Grellmann, Wajih Al-Soufi, Ulrike Baron, Alexander Eychmueller, and Thomas Thomsen

Physikalisch-Chemische Trenn- und Messmethoden 22, 109-18 (1990)

Instabilities and chaotic emission of far infrared ammonia lasers

W. Klische, C. O. Weiss, W. Al-Soufi, G. Huettmann

Cambridge Studies in Modern Optics 1986, 4, 237–239

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