ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Hours of tutorials: 1 Expository Class: 26 Interactive Classroom: 14 Total: 41
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology, Functional Biology
Areas: Biochemistry and Molecular Biology, Plant Physiology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
Know the basic concepts, terminology and methodology of Genetic Engineering and Biotechnology.
Understand the different applications of Biotechnology.
Manage the specialized bibliography and present works related to Genetic Engineering and Biotechnology.
PROGRAM AND DURATION OF LECTURES (FACE-TO-FACE) (26h)
LESSON 1. INTRODUCTION TO GENETIC ENGINEERING AND BIOTECHNOLOGY (1h)
LESSON 2. RECOMBINANT DNA ENZYMOLOGY. (2h)
LESSON 3. NUCLEIC ACID AND PROTEINS HYBRIDIZATION. (2h)
LESSON 4. DNA CLONING IN E. coli (3h)
LESSON 5. LIBRARIES AND MICROARRAYS. (1h)
LESSON 6. PCR. (2h)
LESSON 7. DNA SEQUENCING. (2h)
LESSON 8. DIRECTED SITE MUTAGENESIS AND PROTEIN ENGINEERING. (2h)
LESSON 9. EXPRESSION OF RECOMBINANT PROTEINS IN E. coli. (2h)
LESSON 10. GENETIC ENGINEERING WITH YEAST. (1h)
LESSON 11. DNA CLONING IN ANIMAL CELLS. (1h)
LESSON 12. BIOTECHNOLOGY IN ANIMALS AND BIOMEDICAL APPLICATIONS. (2h)
LESSON 13. INTRODUCTION TO PLANT BIOTECHNOLOGY. (0.5h)
LESSON 14. IN VITRO CULTURE, SOMATIC EMBRYOGENESIS AND MICROPROPAGATION. (1.5h)
LESSON 15. METHODS OF GENETIC TRANSFORMATION OF PLANTS. (1.5h)
LESSON 16. APPLICATIONS OF PLANT BIOTECHNOLOGY. (1.5h)
PROGRAM OF LABORATORY PRACTICE (FACE-TO-FACE) (8h)
-PRACTICE 1. DNA digestion with restriction enzymes. Purification of genomic DNA from saliva; PCR amplification of a region of the purified genome (2.5h).
-PRACTICE 2. Agarose gel analysis of DNA fragments of restriction and PCR products. Assessment of the results obtained in the two sections (2.5h).
-PRACTICE 3. Preparation of culture medium, asepsis of plant material and introduction in vitro (3h).
SEMINAR PROGRAM (FACE-TO-FACE) (6h)
-Seminar 1. Labeling techniques of hybridization probes (1h)
-Seminar 2. Variations of the basic technique of PCR and its applications (1h)
-Seminar 3. DNA sequencing techniques. (1h)
-Seminar 4. Realization of problems of the matter (1h)
-Seminar 5. Realization of problems of the matter (1h)
-Seminar 6. Realization of problems of the matter (1h)
PROGRAM OF THE SMALL GROUP TUTORING FACE-TO-FACE) (1h)
-Tutorial 1. Detailed presentation of the teaching guide and interactive activities. (1h)
EXAM (2h)
Mandatory face-to-face written exam of the contents of lectures and seminars.
Basic
-Brown, T.A., 2016. Gene cloning and DNA analysis: An Introduction. 7th ed. Chichester: Wiley-Blackwell.
-Clark, D.P. and Pazdernik, N.J., 2016. Biotechnology. [on line] 2nd ed. Amsterdam: Elsevier Academic Press. Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
-Perera, J., Tormo, A. y García, J.L., 2002. Ingeniería genética. 2 v. Madrid: Síntesis.
-Real García, M.D., Rausell Segarra, C. y Latorre Castillo, A., 2017. Técnicas de ingeniería genética. Madrid: Síntesis.
-Stewart jr, C.N. (Ed), 2016. Plant biotechnology and genetics: principles, techniques, and applications. 2nd ed. Hoboken: John Wiley & Sons Inc.
Complementary
-Glick, B.R. and Patten, C.L., 2017. Molecular biotechnology: principles and applications of recombinant DNA. 5th ed. Washington DC: ASM Press.
-Green, M.R. and Sambrook, J., 2012. Molecular cloning: a laboratory manual. 4th ed. 3 v. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
-McPherson, M.J. and Møller, S., 2006. PCR. 2nd ed. New York: Taylor & Francis.
-Renneberg, R., Berkling, V., and Loroch, V., 2016. Biotechnology for beginners [on line]. Amsterdam: Elsevier. Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128012246/biotech…
-Chrispeels, M.J., and Gepts, P. 2017. Plant, Genes, and Agriculture. Sustainability through Biotechnology. Oxford: Oxford University Press.
Another resources
- http://biomodel.uah.es. Website of Universidad Alcalá de Henares
-https://www.jove.com/science-education-library. Vídeos educativos sobre diferentes técnicas de bioloxía molecular e enxeñaría xenética.
- http://www.ncbi.nlm.nih.gov/guide/. The National Center for Biotechnology Information, (NCBI) main source of databases (nucleotides, genes and genomes) and bioinformatics tools.
-Electronic resources of BUSC: Cell, Nature, Science, Sciencedirect, etc.
The subject of Genetic Engineering participates, in greater or lesser extent, in the acquisition by the student of the basic and general competencies that appear in the Biology Degree Report.
The specific skills that develop in this area, as recounted in the notes grade in Biology are as follows:
CE3 - Know and know how to apply instrumental techniques, and design work protocols in the laboratory and in the field, applying the regulations and appropriate techniques related to safety, hygiene, waste management and quality.
CE8 - Understand the principles of bioenergetics and biosignaling, and know the main metabolic pathways and their regulation, as well as the flow of genetic and biochemical information in living beings.
CE11 - Understand and integrate the functioning and regulation of the main physiological processes of living beings, as well as their interaction with the biotic and abiotic environment.
The subject of Genetic Engineering participates, in greater or lesser extent, in the acquisition by the student of the following transversal competences:
CT1 - Ability to search, process, analyze and synthesize information from various sources.
CT2 - Capacity for reasoning, argumentation and critical thinking.
CT3 - Ability to work in a group and deal with problematic situations collectively.
CT4 - Ability to prepare and present an organized and understandable text.
CT5 - Ability to make a public presentation in a clear, concise and coherent way.
CT7 - Ability to apply ICTs in the field of Biology.
CT8 - Ability to solve problems through the integrated application of their knowledge, promoting initiative and creativity.
CT9 - Ability to organize and plan work.
CT10 - Ability to interpret experimental results.
Content development and mandatory attendance
Paragraph CONTENTS OF THE MATTER shows the approximate durations of the different activities of students in the classroom or laboratory.
a) Theory subject program will be presented by Professor lecture format in the lectures, which are not compulsory for students. Will last approximately 1hour. In the lectures, in addition to waxing, it will use audio-visual presentations, which will be accessible from the virtual course, to make it easier to track the contents. In classes, students can disrupt the teacher's explanations with questions or concerns, as well as the teacher can ask questions and request participation of learners in discussion and presentation of content and require finding information for delivery in the next class. Lectures require daily follow-up and dedication (in the classroom and outside it) because the teacher will present the basics of the subject that must be deepened through the management of the literature and study. It will work, in general, all the powers and objectives of subject, through the interplay of content and concepts of matter, and familiarization and handling of the main bibliographical sources in the field of genetic engineering and biotechnology to enable the student track and deepening the information received in the classroom.
b) The laboratory practices will consist of three compulsory sessions (except for the repeaters that they have already overcome) in the laboratory. The practices are of obligatory attendance to 100% (a list of signatures will be taken). Practices consist in performing some of the basic techniques in genetic engineering and will serve to reinforce and see the actual implementation of the contents displayed on the theory program. Students must sign a listing of the group. The students must carry out practical reports with the presentation and discussion of the results obtained, as well as answer theoretical/practical questions (present in the practical script itself available on the Virtual Campus) related to the laboratory practices carried out (both required). These questionnaires are designed to reinforce deep learning of the subject, the interrelationship of concepts, learning to transfer and apply knowledge, and practicing critical thinking of scientific methodology. It will be necessary to deliver a memory-questionnaire for practices 1 and 2 and another corresponding to practice 3.
c) Seminars 1-3 in the classroom will consist of the explanation by the teaching staff of a series of specific Genetic Engineering techniques related to the topics previously covered in expository classes. Previously, information (figures, diagrams, bibliography, etc.) will be left to the students in the Virtual Campus on the topics to be discussed so that they can prepare the questions on which they will be questioned through interventions in the classroom or by completing questionnaires of answers.
Seminars 4-6 will consist of the resolution in the classroom, in working groups or individually, of problems and questions proposed by the teaching staff. Books and any other teaching and computer material may be used for resolution, at the discretion of the teacher. The response bulletins will be delivered, at the end of the classroom session, for evaluation. They do not require more remote work than keeping the study of the subject up to date.
All the seminars are of voluntary attendance and realization, and evaluable (through the answers in the classroom, completion of questionnaires and delivery of problems bulletins).
d) The small group tutorial program is shown in the Contents section of this guide and they are not compulsory or evaluable.
e) The exam is a compulsory face-to-face test for the evaluation of knowledge and skills using a written test in which multiple choice questions, problems similar to those of seminars 4-6 and development questions will be formulated, covering the topics covered in the expository classes. and in seminars.
Qualification of the student is a weighted average of the performance in the parts where their work is evaluated: exam, participation and answers in the seminars and report -questionnaire of practices. The same tools will be used to assess competencies according to the following scheme:
- With the examination the following skills are assessed: CE3, CE8, CE11, CB1, CB2, CB4, CG1, CG2, CG4, CG5, CT1, CT2, CT4 and CT8.
- In the seminars: CE3, CE8, CE11, CB1, CB2, CB4, CG1, CG2, CG3, CG4, CG5, CT1, CT2, CT3, CT5, CT7, CT8 and CT10.
- In the laboratory practices reports-questionnaires: CE3, CE8, CE11, CB1, CB2, CB4, CB5, CG1, CG2, CG3, CG4, CG5, CT1, CT2, CT3, CT4, CT7, CT8, CT9 and CT10.
Common standards in the evaluation in both calls.
The continuous evaluation will suppose 35% of the final grade, distributed in 15% the answer bulletins and questionnaires of the seminars (voluntary) and in 20% of the reports-questionnaires of practices, distributed as follows: 12.5% practices 1 and 2, and 7.5% practice 3. Both are mandatory.
There will be a compulsory face-to-face final exam that represents 65% of the final grade. The contents evaluated in the exam includes the contents of the expository classes and the seminars.
In the second evaluation call, only the compulsory face-to-face final exam will be repeated (which will also represent 65% of the final grade), keeping the grade obtained in the continuous evaluation.
The final exam is considered as a compulsory complementary test to continuous assessment for all students. It will be necessary to achieve a minimum of 4 out of 10 in the final exam (in the two evaluation opportunities) for the continuous assessment to be added. Otherwise, the final grade will be exclusively that of the exam. A minimum final grade of 5 out of 10 will be necessary to pass the course.
There will be no compensatory mechanisms for the non-attendance and / or performance of some mandatory and/or evaluable activity.
Those learners not meeting the required attendance requirements and/or not engage all mandatory activities are not entitled to examination, and therefore cannot pass the subject in either assessment opportunities progress.
The 100% assistance activities per se will not score in the final grade.
Repeating students: repeating students will be able to keep the note from the two previous years of the different sections of the continuous assessment. In the case of the practice memory-questionnaire, it will be necessary to have reached a minimum of 5 out of 10 to be able to keep the grade. Otherwise, the obligatory practices and the delivery of the reports-questionnaires must be repeated. The conservation of the note of the seminars implies its entire set. In any case, all the repeaters will have to take the mandatory final exam (contents of expository and seminars). The weighting of each section for the final grade will be the same as that indicated above.
Plagiarism and improper use of technologies in the performance of tasks or tests: in cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and review of grades will apply.
The distribution by different means (for example, on web pages, distribution to people outside the course, etc.) without the consent of the teaching staff, of any teaching material available in the virtual classroom is also strictly prohibited.
IN CLASSROOM WORK (Total: 43h)
Lectures: 26h
Laboratory: 8h
Seminars: 6h
Tutoring in small groups: 1h
Examinations: 2h
STUDENT'S PERSONAL WORK (Total: 69.5 h)
Individual Study: 49h
Development of practice reports and theoretical/practical questionnaires: 7h
Development of group work: 13.5 h
The most important recommendation is that attendance and use of classes is essential for this system. Reaching the final exam without knowing the teacher and to pass the subject is impossible given the compulsory attendance in some activities. Bring up the subject, practice thinking (correlating concepts) and deepen the knowledge shown in class is essential to pass the subject.
In the written tests is important to read the questions, interpret the logic of the results or answers, to use precise and rigorous language, and review answers.
Quality and breadth of literature reviewed (if applicable), structure of the answers and presentation (spelling, images, diagrams themselves, etc.), adequacy of the required contents, clarity of exposition and mastery of language and scientific concepts will be assessed in laboratory practices report-theoretical/practical questionnaire.
Although they are not mandatory, it is highly recommended to attend and prepare the seminars well, completing the evaluable tests that they represent. Not only do they represent an important part of the final evaluation, but they also allow you to review the expository material, and its contents are also exam material.
Make use of the tutorials and contact the teaching staff to solve any doubts and problems that arise in the study of the subject and to monitor the learning.
Communication with teachers
There will be an active virtual classroom of the subject through the Virtual Campus of the USC.
Face-to-face communication in the classroom and in the office. Communications may also be established through institutional email, via the MS-Teams platform, messages through the Virtual Campus forum and by telephone.
Plagiarism and improper use of technologies in the performance of tasks or tests: in cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and review of grades will apply.
he distribution by different means (for example, on web pages, distribution to people outside the course, etc.) without the consent of the teaching staff, of any teaching material available in the virtual classroom is also strictly prohibited.
Guillermo Covelo Artos
Coordinador/a- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816930
- guillermo.covelo [at] usc.es
- Category
- Professor: Temporary PhD professor
Miguel González Blanco
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881815386
- miguel.gonzalez.blanco [at] usc.es
- Category
- Professor: University Lecturer
María Crugeiras Ríos
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- maria.crugeiras.rios [at] usc.es
- Category
- Xunta Pre-doctoral Contract
Marcos Viejo Somoano
- Department
- Functional Biology
- Area
- Plant Physiology
- Phone
- 881813322
- marcos.viejo [at] usc.es
- Category
- Professor: LOU (Organic Law for Universities) PhD Assistant Professor
| Wednesday | |||
|---|---|---|---|
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Classroom 06. Diane Fosey and Jane Goodall |
| Thursday | |||
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Classroom 06. Diane Fosey and Jane Goodall |
| Friday | |||
| 09:00-10:00 | Grupo /CLIS_01 | Spanish | Classroom 06. Diane Fosey and Jane Goodall |
| 05.29.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 05.29.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
| 07.09.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |