ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.2 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.45
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Functional Biology
Areas: Plant Physiology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
- Getting a solid preparation of students, both theoretical and practical, in Biotechnology applied to plants.
- Know the general concepts of biotechnological techniques and relating them to other basic and specific subjects of Degree in Biology.
- Understand the physiological basis and methodology of plant propagation by culture in vitro, and its practical applications in the industry today.
- Gain knowledge on the genetic modification of plants, methods, applications and evaluation of them.
- Students should reach basic theoretical and practical knowledge in the field for possible application in the labor market.
Scenarios 2 and 3: unchanged.
THEORY PROGRAM. Lectures (26 HOURS)
THEORY PROGRAM. Lectures (26 HOURS)
Unit 1 (2h). Introduction to de Biotechnology. Historical background and current applications.
Unit 2 (1h). Cell growth and differentiation. Totipotency. Direct and indirect organogenesis. Somaclonal variation and instability of cultures.
Unit 3 (3h).Introduction to in vitro culture. Establishment in aseptic culture. Cuture conditions and growth media. Plant material: selection and sterilizarion.
Unit 4 (1h). Micropropagation: Multiplication, rooting and acclimatization. In vitro culture of of apical meristems. Production of virus-free plants.
Unit 5 (1h). Somatic embryogenesis. Encapsulation. Artificial seeds.
Unit 6 (1h). Haploid plants. Application of Haploids in Plant Breeding.
Unit 7 (1h). Cell culture. Cell suspensions and plant cell culture secondary metabolites production. Bioreactors culture.
Unit 8 (1h). Protoplats culture: isolation, culture and viability of protoplast. Somatic hybrid. Plant regeneration from protoplasts.
Unit 9 (1h). Gene banks. Biotechnology and ex situ conservation of germplasm. Minimal growth in vitro culture. Cryopreservation.
Unit 10 (1h). The genome of plants. Genetic engineering. Genetic modified organisms.
Unit 11 (3h). Plant transformation vectors. Selectable marker genes and reporter genes.
Unit 12 (2h). Transformation mediated by biological vectors: Agrobacterium tumefaciens, A. rhizogenes and viruses.
Unit 13 (1h). Direct gene transfer. Biolística. Electroporation.
Unit 14 (1h). Regeneration and analysis of transgenic plants. Molecular confirmation methods. Inheritance of characters.
Unit 15 (34). Applications and recent advances in plant genetic engineering. Genetic manipulation of resistance. Transgenic foods. Metabolic engineering. Agricultural biotechnology.
Unit 16 (2h). Biosafety. GMOs risks and legislation.
OBLIGATORY LABORATORY SESSIONS (10 HOURS)
Session 1 (2,5h). In vitro culture media preparation. Sterilization of culture media and additional material.
Session 2 (2h). Preparation and sterilization of the starting plant material. Establishment of initial aseptic cultures.
Session 3 (1h). Working in hoods.
Session 4 (2,5h). Acclimatization of micropropagated plants to ex vitro conditions. Data collection.
Session 5.(2h). Data collection and result presentation.
Fieldwork (2h) (optional): Visit to a Center for Plant Biotechnology (depending on sanitary conditions, finantiation and center availability)
SMALL GROUP TUTORING (3 HOURS)
During the tutorials you will be guided on the resolution of the proposed activities
Scenarios 2 and 3: unchanged.
Scenarios 1 y 2
BASIC BIBLIOGRAPHY
Altman y Hasegawa ed (2012) Plant Biotechnology and agriculture. Prospects for the 21st century. Elsevier
Peña, L. ed., 2005. Transgenic Plants: Methods and Protocols. Totowa: Humana Press.
Slater, A., Scott, N.W. and Fowler, M.R., 2008. Plant Biotechnology: The Genetic Manipulation of Plants. 2nd ed. Oxford: Oxford University Press.
Smith, R.H., 2012. Plant Tissue Culture: Techniques and Experiments. 3rd ed. San Diego: Academic Press.
COMPLEMENTARY
Al-Khayri, JM, Jain MS y Johson DV eds2016. Advances in Plant Breeding Strategies: Agronomic, Abiotic and Biotic Stress Traits Switzerland. Springer.
Collin, H.A. and Edwards, S., 1998. Plant Cell Culture. Oxford: BIOS Scientific Publishers.
Dixon, R.A. and Gonzales, R.A., 1994. Plant Cell Culture: a Practical Approach. 2nd ed. Oxford: IRL Press at Oxford University
Dodds, J.H. and Roberts, L.W., 1995. Experiments in plant tissue culture. 3rd ed. Cambridge: Cambridge University Press.
Gresshoff, P.M. ed., 1997. Technology transfer of Plant Biotechnology. Boca Ratón, FL: CRC Press.
NETWORK RESOURCES
Srivastava, P.S, Narula, A; Srivastava, S and Bhojwani, SS (2004) Plant Biotechnology and Molecular Markers Bostyon Springer Full text available via EBSCOhost Ebooks
In the virtual classroom will include teaching material prepared by the teacher and links to online resources.
Scenario 3
NETWORK RESOURCES
Srivastava, P.S, Narula, A; Srivastava, S and Bhojwani, SS (2004) Plant Biotechnology and Molecular Markers Bostyon Springer Full text available via EBSCOhost Ebooks
In the virtual classroom will include teaching material prepared by the teacher and links to online resources.
Basic competences
They will develop all that are specified in the Report of the Degree in Biology
Specific skills
CE1.c - Scope of competence: instrumental knowledge, skills and abilities. Basic biology techniques. Basics of biotechnology in plants
CE7.d - Competence Scope: Biotechnology and biomedicine: Biotechnology and recombinant DNA: Genetic engineering in plants, transgenic plants and foods.
CE7.f - Competence Scope: Biotechnology and biomedicine; Biomarkers, bio tests and crops. Basic techniques used for plant biotechnology.
CE7.h - Competence Scope: Biotechnology and biomedicine: Agrifood and forestry applications.
Transversal competences
CT1 - Capacity for analysis and synthesis.
CT2 - Capacity for reasoning and argumentation
CT3 - Capacity to work in groups and deal with problems in a collective way
CT4 - Capacity to use adequate, diverse and updated information
CT5 - Ability to develop and present an organized and understandable text
CT6 - Ability to give a speech in public in a clear, concise and coherent way
CT7 - Compromise of veracity for the information given to the scientific community
CT8 - Ability in the management of Information and Communications technologies (ICTs)
CT9 - Use of bibliographic resources and Internet information
CT10 - Use of information provided in a foreign language
CT11 - Ability to solve problems by means of the implementation of an integrated knowledge management.
Scenarios 2 and 3: unchanged.
In support of teaching, a virtual classroom will be available on the Moodle platform of the Virtual Campus, to which teaching material will be uploaded and which will be used as a means of communication with students. The monitoring of the continuous evaluation will be carried out through the delivery of works, the completion of questionnaires ... using the Moodle platform.
Scenario 1
Lectures: synchronously in the classroom
Interactive laboratory classes: 50% of the practices will be carried out synchronously in the laboratory and the other 50% will be asynchronous and telematic. The follow-up of the practices will be carried out through assistance and preparation and delivery of issues; as well as a job. These activities are mandatory and evaluable.
Tutorials: They may be face-to-face or telematic (Teams) at previously established times, or by appointment.
Fieldwork (optional): Visit to a Center for Plant Biotechnology (depending on sanitary conditions, funding and center availability). To make the visit it is necessary to have attended 50% of the lectures.
Scenario 2
Expository classes: The expository classes will be telematic and synchronous, maintaining the official schedule of the classes (except that due to supervening causes they must be asynchronous, a situation that will be communicated beforehand to the students).
Interactive laboratory classes: 50% of the practices will be carried out synchronously in the laboratory and the other 50% will be asynchronous and telematic. The follow-up of the practices will be carried out through assistance and preparation and delivery of issues; as well as a job. These activities are mandatory and evaluable.
Tutorials: They may be face-to-face or telematic (Teams) at previously established times, or by appointment.
Fieldwork (optional) Visit to a Center for Plant Biotechnology: For health reasons, the visit to the center is canceled.
Scenario 3
Expository classes: The expository classes will be telematic and synchronous, maintaining the official schedule of the classes (except that due to supervening causes they must be asynchronous, a situation that will be communicated beforehand to the students).
Interactive laboratory classes: 100% of the practices will telematic, 50% be carried out synchronously and the other 50% will be asynchronous. These activities are mandatory and evaluable.
Tutorials: Telematic (Teams) at previously established times, or by appointment.
Fieldwork (optional) Visit to a Center for Plant Biotechnology: For health reasons, the visit to the center is canceled
Scenarios 1, 2 y 3
PERSONAL WORK OF THE STUDENT
Elaboration of works (19,5h)
Students will write a short report according to the rules of a scientific paper and using the results obtained at practical sessions (individually, 20% final mark).
In addition, students will prepare a work using the knowledge acquired in class, in a collective form (20% final mark).
The assessment criteria are outlined in the table on the following link:
http://www.usc.es/gl/centros/bioloxia/criteriosavaliacion.html
60% of the final mark will match the exam, and the remaining 30% with the continuous assessment system, which will be added only if it reaches 40% of the maximum mark in the exam.
The final grade will be:
a) Final exam (theory and practice)60%
b) Practice report. Complementary to continuous evaluation (20% final mark)
c) Collective work 10%. Complementary to continuous evaluation (20% final mark)
This weighting will be applicable in all three scenarios and for both the first and second chances. In the case of repeating students, the grades obtained in the continuous assessment tests (b and c) will be kept for one year as long as 50% of the maximum grade has been obtained.
Scenarios 2 y 3
If in-person assessment activities that cannot be carried out in this way, and cannot be delayed, will be carried out electronically using the institutional tools in Office 365 and Moodle. In this case, the adoption of a series of measures will be required that will require the student to have a device with a microphone and a camera while adequate evaluation software is not available.
For cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the assessment of two academic performance and the review of qualifications will apply.
The distribution by different means (for example, web pages) and without the teacher's consent, of any teaching material posted in the virtual classroom is also prohibited.
Scerarios 1, 2 y 3
Assessment of competences
Final exam: CT1, CT2, CT5, CE1.c, CE7.d, h,f
Practice report (Individual work): CG2 CT1,CT2,CT5,CT7, CT9,CT10, CT11, CE1.c, CE7.f
Collective work: CB3, CG2, CT2, CT3,CT4, CT6, CT7, CT8, CT9, CT10
WORK IN CLASSROOM (HOURS)
Lectures...............................................26
Laboratory Practice / Fieldwork.............. 12
Small group tutoring................................3
Exam 2
CLASSROOM WORK................................43
-------------------------------------------------- ---
INDIVIDUAL AND COLLECTIVE WORK (HOURS)
Individual study .............................................50
Development of practice report .........................12
Preparation of collective work ........................... 7,5
PERSONAL WORK............................................69.5
Scenarios 2 and 3: unchanged.
• Attend all teaching activities with active participation in them.
• Consultation of recommended bibliography.
• Delving into the issues.
• Make frequent use of tutorials for any issue related to the subject
Scenarios 2 and 3: unchanged.
It is recommended knowledge in Plant Physiology, Genetic, Cell Biology,
CONTINGENCY PLAN before a possible change of scenery
1. Objectives: unchanged
2. Learning outcomes: unchanged
3. Contents: unchanged
4. Bibliographic material:
Scenario 2: unchanged
Scenario 3:
Scenario 3
NETWORK RESOURCES
Srivastava, P.S, Narula, A; Srivastava, S and Bhojwani, SS (2004) Plant Biotechnology and Molecular Markers Bostyon Springer Full text available via EBSCOhost Ebooks
In the virtual classroom will include teaching material prepared by the teacher and links to online resources.
5. Competencies: unchanged
6. Methodology:
In support of teaching, a virtual classroom will be available on the Moodle platform of the Virtual Campus, to which teaching material will be uploaded and which will be used as a means of communication with students. The monitoring of the continuous evaluation will be carried out through the delivery of works, the completion of questionnaires ... using the Moodle platform.
Scenario 1
Lectures: synchronously in the classroom
Interactive laboratory classes: 50% of the practices will be carried out synchronously in the laboratory and the other 50% will be asynchronous and telematic. The follow-up of the practices will be carried out through assistance and preparation and delivery of issues; as well as a job. These activities are mandatory and evaluable.
Tutorials: They may be face-to-face or telematic (Teams) at previously established times, or by appointment.
Fieldwork (optional): Visit to a Center for Plant Biotechnology (depending on sanitary conditions, funding and center availability). To make the visit it is necessary to have attended 50% of the lectures.
Scenario 2
Expository classes: The expository classes will be telematic and synchronous, maintaining the official schedule of the classes (except that due to supervening causes they must be asynchronous, a situation that will be communicated beforehand to the students).
Interactive laboratory classes: 50% of the practices will be carried out synchronously in the laboratory and the other 50% will be asynchronous and telematic. The follow-up of the practices will be carried out through assistance and preparation and delivery of issues; as well as a job. These activities are mandatory and evaluable.
Tutorials: They may be face-to-face or telematic (Teams) at previously established times, or by appointment.
Fieldwork (optional) Visit to a Center for Plant Biotechnology: For health reasons, the visit to the center is canceled.
Scenario 3
Expository classes: The expository classes will be telematic and synchronous, maintaining the official schedule of the classes (except that due to supervening causes they must be asynchronous, a situation that will be communicated beforehand to the students).
Interactive laboratory classes: 100% of the practices will telematic, 50% be carried out synchronously and the other 50% will be asynchronous. These activities are mandatory and evaluable.
Tutorials: Telematic (Teams) at previously established times, or by appointment.
Fieldwork (optional) Visit to a Center for Plant Biotechnology: For health reasons, the visit to the center is canceled
Scenarios 1, 2 y 3
PERSONAL WORK OF THE STUDENT
Elaboration of works (19,5h)
Students will write a short report according to the rules of a scientific paper and using the results obtained at practical sessions (individually, 20% final mark).
In addition, students will prepare a work using the knowledge acquired in class, in a collective form (20% final mark).
7. Assessment system
The assessment criteria are outlined in the table on the following link:
http://www.usc.es/gl/centros/bioloxia/criteriosavaliacion.html
60% of the final mark will match the exam, and the remaining 30% with the continuous assessment system, which will be added only if it reaches 40% of the maximum mark in the exam.
The final grade will be:
a) Final exam (theory and practice)60%
b) Practice report. Complementary to continuous evaluation (20% final mark)
c) Collective work 10%. Complementary to continuous evaluation (20% final mark)
This weighting will be applicable in all three scenarios and for both the first and second chances. In the case of repeating students, the grades obtained in the continuous assessment tests (b and c) will be kept for one year as long as 50% of the maximum grade has been obtained.
Scenarios 2 y 3
If in-person assessment activities that cannot be carried out in this way, and cannot be delayed, will be carried out electronically using the institutional tools in Office 365 and Moodle. In this case, the adoption of a series of measures will be required that will require the student to have a device with a microphone and a camera while adequate evaluation software is not available.
For cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the assessment of two academic performance and the review of qualifications will apply.
The distribution by different means (for example, web pages) and without the teacher's consent, of any teaching material posted in the virtual classroom is also prohibited.
Scerarios 1, 2 y 3
Assessment of competences
Final exam: CT1, CT2, CT5, CE1.c, CE7.d, h,f
Practice report (Individual work): CG2 CT1,CT2,CT5,CT7, CT9,CT10, CT11, CE1.c, CE7.f
Collective work: CB3, CG2, CT2, CT3,CT4, CT6, CT7, CT8, CT9, CT10
8. Study time and individual work: unchanged
9. Recommendations for the study of the subject: unchanged
Margarita Lema Marquez
- Department
- Functional Biology
- Area
- Plant Physiology
- Phone
- 881813342
- margarita.lema [at] usc.es
- Category
- Professor: Temporary supply professor for IT and others
| Monday | |||
|---|---|---|---|
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
| Tuesday | |||
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
| Wednesday | |||
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
| 06.14.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 07.20.2021 10:00-14:00 | Grupo /CLE_01 | Classroom 05 (video-conference). Rita Levi Montalcini |
| 07.20.2021 10:00-14:00 | Grupo /CLE_01 | Classroom 06. Diane Fosey and Jane Goodall |