ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.2 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.45
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology
Areas: Biochemistry and Molecular Biology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
The goal is that learning outcome of this matter by alumni to enable them:
-Understand the concepts, terminology and basic methodology of genetic engineering and some of its applications.
-Manage the literature and present work related to the Genetic Engineering and Biotechnology.
All the contents of the different activities will be the same in the different possible teaching scenarios.
PROGRAM AND DURATION OF LECTURES (FACE-TO-FACE) (26h)
LESSON 1. INTRODUCTION TO GENETIC ENGINEERING AND BIOTECHNOLOGY (1.5h)
LESSON 2. RECOMBINANT DNA ENZYMOLOGY. (3h)
LESSON 3. NUCLEIC ACID AND PROTEINS HYBRIDIZATION. (2h)
LESSON 4. DNA CLONING IN E. coli (3h)
LESSON 5. LIBRARIES AND MICROARRAYS. (1.5h)
LESSON 6. PCR. (2h)
LESSON 7. DNA SEQUENCING. (2h)
LESSON 8. DIRECTED SITE MUTAGENESIS AND PROTEIN ENGINEERING. (2h)
LESSON 9. EXPRESSION OF RECOMBINANT PROTEINS IN E. coli. (2.5h)
LESSON 10. GENETIC ENGINEERING WITH YEAST. (1.5h)
LESSON 11. DNA CLONING IN ANIMAL CELLS. (2h)
LESSON 12. BIOTECHNOLOGY IN ANIMALS AND BIOMEDICAL APPLICATIONS. (2h)
LESSON 13. BIOTECHNOLOGY IN PLANTS. (1h)
PROGRAM OF LABORATORY PRACTICE (FACE-TO-FACE) (5h)
In teaching scenario 3, practices 1 and 2 will also be done electronically.
Face-to-face practices
-PRACTICE 1. Part A. DNA digestion with restriction enzymes (1h 15').
Part B. Purification of genomic DNA from saliva; PCR amplification of a region of the purified genome (1h 15 ').
-PRACTICE 2. Part A. Agarose gel analysis of DNA fragments of restriction (1h 15').
Part B. Analysis and evaluation of the results obtained in the two practices (1h 15 ’).
SEMINAR PROGRAM (FACE-TO-FACE) (5h)
-Seminar 1. Labeling techniques of hybridization probes (1h)
-Seminar 2. Variations of the basic technique of PCR and its applications (1h)
-Seminar 3. DNA sequencing techniques. (1h)
-Seminar 4. Realization of problems of the matter (2h)
PROGRAM OF THE SMALL GROUP TUTORING (5h)
-Tutorial 1. (Face-to-face) Presentation to students of the work of seminars to be made by each group throughout the course, detailed presentation of the teaching guide. (1h)
-Tutorials 2, 3, 4 and 5. (Face-to-face or telematics) Resolution of doubts and questions about the lectures, seminars and practices. They will be carried out at the request of the students. (1h each)
Basic
-Brown, T.A., 2016. Gene cloning and DNA analysis: An Introduction. 7th ed. Chichester: Wiley-Blackwell.
-*Clark, D.P. and Pazdernik, N.J., 2016. Biotechnology. [on line] 2nd ed. Amsterdam: Elsevier Academic Press. Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
-Perera, J., Tormo, A. y García, J.L., 2002. Ingeniería genética. 2 v. Madrid: Síntesis.
-Real García, M.D., Rausell Segarra, C. y Latorre Castillo, A., 2017. Técnicas de ingeniería genética. Madrid: Síntesis.
Further reading
-*Clark, D.P, 2019. Molecular Biology. [on line] 3rd ed. Amsterdam: Elsevier . Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128132883/molecul…
-Glick, B.R. and Patten, C.L., 2017. Molecular biotechnology: principles and applications of recombinant DNA. 5th ed. Washington DC: ASM Press.
-Green, M.R. and Sambrook, J., 2012. Molecular cloning: a laboratory manual. 4th ed. 3 v. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
-McPherson, M.J. and Møller, S., 2006. PCR. 2nd ed. New York: Taylor & Francis.
-*Renneberg, R., Berkling, V., and Loroch, V., 2016. Biotechnology for beginners [on line]. Amsterdam: Elsevier. Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128012246/biotech…
Another resources
- http://biomodel.uah.es. Web Universidad Alcalá de Henares
-https://www.jove.com/science-education-library. Vídeos educativos sobre diferentes técnicas de bioloxía molecular e enxeñaría xenética.
- http://www.ncbi.nlm.nih.gov/guide/. The National Center for Biotechnology Information, (NCBI) main source of databases (nucleotides, genes and genomes) and bioinformatics tools.
-Electronic resources of BUSC: Cell, Nature, Science, Sciencedirect, etc.
The subject of Genetic Engineering participates, in greater or lesser extent, in the acquisition by the student of the basic and general competencies that appear in the Biology Degree Report.
The specific skills that develop in this area, as recounted in the notes grade in Biology are as follows:
CE1.c - Sphere of competence of instrumental knowledge, skills and abilities:
-Basic techniques in biology
CE2.e - Sphere of competence of origin and molecular basis of life:
-Analysis of biomolecules and metabolic processes.
CE2.f - Sphere of competence of origin and molecular basis of life:
-Molecular, cellular and tissue analysis,
CE7.d - Sphere of competence of biotechnology and biomedicine:
- Biotechnology and recombinant DNA.
CE7.g Sphere of competence of biotechnology and biomedicine:
-Methods of clinical and molecular analysis.
CE7.h - Biotechnology and recombinant DNA.
-Agri-food and forestry applications.
The subject of Genetic Engineering participates, in greater or lesser extent, in the acquisition by the student of the following transversal competences:
CT1 - Ability to analyze and synthesize.
CT2 - Ability to reason and argument.
CT3 - Ability to work together and address problematic situations collectively.
CT4 - Ability to obtain adequate information, diverse and updated.
CT5 - Ability to develop and present an organized and understandable text.
CT6 - Ability to make a public display in a clear, concise and consistent.
CT7 - Commitment to accuracy of the information provided to others.
CT8 - Skill in the use of ICT.
CT9 - Use of bibliographic and Internet.
CT10 - Use information in foreign language.
CT11 - Ability to solve problems through the integrated application of knowledge.
CONTENT DEVELOPMENT AND MANDATORY ATTENDANCE
Paragraph CONTENTS OF THE MATTER shows the approximate durations of the different activities of students in the classroom or laboratory.
a) Theory subject program will be presented by Professor lecture format in the lectures, which are not compulsory for students. Will last approximately 1hour. In the lectures, in addition to waxing, it will use audio-visual presentations, which will be accessible from the virtual course, to make it easier to track the contents. In classes, students can disrupt the teacher's explanations with questions or concerns, as well as the teacher can ask questions and request participation of learners in discussion and presentation of content and require finding information for delivery in the next class. Lectures require daily follow-up and dedication (in the classroom and outside it) because the teacher will present the basics of the subject that must be deepened through the management of the literature and study. It will work, in general, all the powers and objectives of subject, through the interplay of content and concepts of matter, and familiarization and handling of the main bibliographical sources in the field of genetic engineering and biotechnology to enable the student track and deepening the information received in the classroom.
In the virtual course of the subject there will be available questionnaires of different thematic blocks of the contents of the expository classes for the self-evaluation of the students. These questionnaires will be voluntary and not evaluable for the final grade.
b) The laboratory practices will consist of two compulsory sessions (except for the repeaters) (2h30 ' each) in the laboratory. The practices are of obligatory attendance to 100% (a list of signatures will be taken). Practices consist in performing some of the basic techniques in genetic engineering and will serve to reinforce and see the actual implementation of the contents displayed on the theory program. Students must sign a listing of the group. Learners must make a laboratory practice report with the presentation and discussion of results and respond to a theoretical /practical questionnaire (present in the practical script available on the Virtual Campus) related to conducted/studied laboratory practice (both mandatory and performed by the couple working in the laboratory). This questionnaire is designed to enhance deep learning of the matter, the interrelation of concepts, learn how to transfer and apply knowledge and practice critical thinking and scientific methodology. Must be delivered, in the same document that the report of practices, for evaluation and will be corrected in a tutorial. Estimated non-contact time of practice report and questionnaire preparation is 7,5 hours.
c) The classroom seminars 1, 2 and 3 will include mandatory presentations of students, in periods of about 10' by working group (5 people maximum), from different parts of the seminar concerning the specific topic previously agreed with Professor and supervised by it in the tutorials. In the Virtual Campus, the specific themes and basic figures for carrying out each work will be available for each work group. Each group, in every seminar, must give the teacher a summary of his presentation. This summary, together with the oral presentation itself, and resolution of questions that arise, will be assessed for the final grade. Teacher will choose at the time, in each case, which member or members of the group will present its section. Posted content on the seminars will be added to the theory program contents of the lectures for the exam.
The works (questionnaires, in the case of scenario 3) from seminars 1 to 3 once corrected by the teacher will be uploaded, after 1 or 2 weeks, to the Virtual Campus so that the students can check their mistakes, review the advice for improvement, see their evolution and that serve as feedback and self-evaluation.
The classroom seminars 4 and 5 will consist in the resolution, by the previous working groups, of problems proposed by the teacher. Books and any other teaching and computer material may be used for their resolution, at the teacher's discretion. The questionnaires solved after the session will be delivered to the teacher for evaluation. It does not require more non-face-to-face work than carrying the study of the subject up to date.
All seminars are mandatory attendance(signature sheet) and completion (delivery of works and bulletin of problems) , with a minimum of 80% and availables. Estimated non-attendance time of development of work exposure/questionnaires (3 per group work throughout the course) is 15.5 hours.
d) The program of small group tutoring is shown in the Contents section of this guide and these are not mandatory or evaluable. The first tutorial will be face-to-face at the beginning of the semester following a calendar agreed with the students. The rest of the tutorials, at the request of the students, may be in person or by telematic mechanisms (synchronous on MS-Teams, asynchronous on Virtual Campus and institutional email).
Qualification of the student is a weighted average of the performance in the parts where their work is evaluated: exam (55%), performing the work of seminars (25%), report -questionnaire of practices (15%) and active participation in class (5%). The same tools will be used to assess competencies according to the following scheme:
- With the examination the following skills are assessed: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CE7.h, CB1, CB2, CB4, CB5, CG1, CG3, CG4, CG5, CT1, CT2, CT4, CT5, CT9, CT10 and CT11.
- In the completion and presentation of works: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CB1, CB3, CB4, CB5, CG1, CG4, CG5, CT1, CT2, CT3, CT4, CT5, CT6, CT7, CT8, CT9 and CT10.
- In the laboratory practices report-questionnaire: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CB1, CB2, CB4, CB5, CG1, CG2, CG3, CG4, CG5, CT1, CT2, CT3, CT5, CT8, CT9 and CT11.
- With the active participation in the classroom: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CE7.h, CB1, CB2, CB3, CB4, CB5, CG4, CG5, CT1, CT2, CT4, CT6, CT7, CT9 and CT11.
The continuous evaluation will suppose 45% of the final grade, distributed in 25% the obligatory works carried out for the seminars, 15% of the compulsory memory-questionnaire of practices and 5% for the expositions and the active participation in the classroom (seminar questionnaire 4).
There will be a compulsory face-to-face final exam that represents 55% of the final grade. The subject evaluated in the exam includes the contents of the expository classes and the seminars.
In the second evaluation call, only the compulsory face-to-face final exam will be repeated (which will also represent 55% of the final grade), keeping the grade obtained in the continuous evaluation.
The final exam is considered as a compulsory complementary test to continuous assessment for all students. It will be necessary to achieve a minimum of 4 out of 10 in the final exam (in the two evaluation opportunities) for the continuous assessment to be added. Otherwise the final grade will be exclusively that of the exam. A minimum final grade of 5 out of 10 will be necessary to pass the course.
There will be no compensatory mechanisms for the non-attendance and / or performance of some mandatory and/or evaluable activity.
Those learners not meeting the required attendance requirements and/or not engage all mandatory activities are not entitled to examination, and therefore cannot pass the subject in either assessment opportunities progress.
The 100% assistance activities per se will not score in the final grade.
Repeating students: repeating students may keep the mark of up to two previous courses of the different sections of the continuous assessment as long as a minimum of 5 out of 10 has been reached, so that they will not be obliged to repeat these activities either and only they will have to do the compulsory final exam.
Plagiarism and improper use of technologies in the performance of tasks or tests:in cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and review of grades will apply.
The distribution by different means (for example, on web pages, distribution to people outside the course, etc.) without the consent of the teaching staff, of any teaching material available in the virtual classroom is also strictly prohibited.
IN CLASSROOM WORK (Total: 43h)
Lectures: 26h
Laboratory: 2,5h
Seminars: 5h
Tutoring in small groups: 5h
Examinations: 2h
STUDENT'S PERSONAL WORK (Total: 69.5 h)
Individual Study: 49h
Development of practice report and theoretical/practical questionnaire: 7,5h
Development of group work: 15.5 h
The most important recommendation is that attendance and use of classes is essential for this system. Reaching the final exam without knowing the teacher and to pass the subject is impossible given the compulsory attendance in some activities. Moreover, even with a 10 on the exam is impossible to pass the course and that weights 55% and there are various classroom and non-contact activities. Bring up the subject, practice thinking (correlating concepts) and deepen the knowledge shown in class is essential to pass the subject.
In the written tests is important to read the questions, interpret the logic of the results or answers, to use precise and rigorous language, and review answers.
Quality and breadth of literature reviewed (if applicable), structure of the answers and presentation (spelling, images, diagrams themselves, etc.), adequacy of the required contents, clarity of exposition and mastery of language and scientific concepts will be assessed in work seminars and laboratory practices report-theoretical/practical questionnaire.
Likewise, it is recommended to review the test made throughout the course, after evaluation of them (it allows verification and correct errors) and consult with the teacher in tutorials any doubts that may arise.
Make use of the tutorials and contact the teaching staff to solve any doubts and problems that arise in the study of the subject and to monitor the learning.
CONTINGENCY PLAN
Teaching methodology
Scenario 2 (distancing; partial restrictions on attendance). The expository classes will be synchronous virtual using the MS-Teams platform, while the seminars will be face-to-face following the official schedule and calendar of the subject. In the case of establishing shifts to attend the seminars, students not present in the classroom will follow the telematic transmission of the classes through the mechanisms enabled by the Faculty Dean's Office. Given the small number of regular students in the subject and the fact that the groups of seminars are even smaller, it is expected that all the students can attend in person at all the seminars.
Laboratory practices will be 50% face-to-face, and will be carried out following the calendar and the groups set by the Faculty Dean's Office, in two sessions of 1.15h that involve part A of each practice. The rest of the laboratory practices require the asynchronous visualization and study of the materials that the professors contribute in the Virtual Campus of the subject. The set of laboratory activities will be evaluated with the obligatory preparation of the practice memory-questionnaire (prepared individually or by pairs of students) that will be delivered in printed format or through the Virtual Campus.
The first tutorial will preferably be face-to-face (if this is not possible, the MS-Teams platform would be used) at the beginning of the semester following a calendar agreed with the students. The rest of the tutorials, at the request of the students, may be in person or by telematic mechanisms to be agreed with the students (MS-Teams platform, Virtual Campus and institutional email).
This teaching planning in scenario 2 will be maintained as long as your organization is compatible with the adoption of the measures established in the health protection protocols of the University of Santiago de Compostela in force at all times.
Scenario 3 (closure of the facilities; impossibility of teaching in person). All teaching activity will be absent. The expository classes will be of synchronous type using the class videos and the presentations used in the classroom plus the complement files (text, videos, audios, etc.) for each topic or group of topics that the teacher contributes in the Virtual Campus.
The seminars will be held asynchronously by working groups. Seminars 1, 2 and 3 will consist of the preparation, in small work groups, of different parts of the seminar concerning the specific topic previously agreed with the teacher and supervised by the teacher in the tutorials and consultations they request, and the subsequent resolution of questionnaires on the specific theme that will be available on the Virtual Campus. Seminar 4 will consist of solving the same problems and questions in the same working groups (the same for all the students). For all the seminars, the student response bulletins must be uploaded on a specific date to the virtual course, in the section enabled for this purpose, for subsequent evaluation.
The entire set of laboratory practices requires the visualization and study of the materials that teachers provide in the Virtual Campus of the subject, which will be necessary for the compulsory completion of the memory-practice questionnaire, carried out individually or in pairs of students, which will be evaluated.
For the first tutorial, a detailed presentation of the contents and development of the course will be uploaded to the Virtual Campus for asynchronous consultation, which would be used in the classroom if it had been attended. The rest of the tutorials, at the request of the students, will be through telematic mechanisms to be agreed with the students (virtual campus and institutional email).
Evaluation system
Scenario 2 (distancing; partial restrictions on attendance). The evaluation mechanism is the same as in scenario 1.
Scenario 3 (closure of the facilities; impossibility of teaching in person). The continuous evaluation will suppose 45% of the final grade, distributed in 30% the obligatory questionnaires made for the seminars and 15% of the memory-obligatory practice questionnaire. Both the questionnaires and the practice memory-questionnaire will be delivered electronically through the Virtual Campus.
There will be a compulsory telematic final exam synchronous through the Virtual Campus that represents 55% of the final grade. The subject evaluated in the exam includes the contents of the expository classes and the seminars.
In the second evaluation call, only the compulsory final exam will be repeated (which will also represent 55% of the final grade), keeping the grade obtained in the continuous evaluation.
Common rules to all scenarios
In all scenarios, the final exam is considered as a compulsory complementary test to continuous assessment for all students. In all scenarios too, it will be necessary to achieve a minimum of 4 out of 10 in the final exam (in the two exams) for the continuous assessment to be added. Otherwise the final grade will be exclusively that of the exam. A minimum final grade of 5 out of 10 will be necessary to pass the course.
There will be no compensatory mechanisms for the non-attendance and / or performance of some mandatory and/or evaluable activity.
Those learners not meeting the required attendance requirements and/or not engage all mandatory activities are not entitled to examination, and therefore cannot pass the subject in either assessment opportunities progress.
The 100% assistance activities per se will not score in the final grade.
Repeating students: repeating students may keep the mark of up to two previous courses of the different sections of the continuous assessment as long as a minimum of 5 out of 10 has been reached, so that they will not be obliged to repeat these activities either and only they will have to do the compulsory final exam.
Plagiarism and improper use of technologies in the performance of tasks or tests: in cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and review of grades will apply.
The distribution by different means (for example, on web pages, distribution to people outside the course, etc.) without the consent of the teaching staff, of any teaching material available in the virtual classroom is also strictly prohibited.
Bibliography
In scenario 3, the recommended bibliography is open access books marked with *, as well as the materials provided by the teacher and those mentioned under "Other resources".
Recommendations for the study of the subject in scenarios 2 and 3.
To the aforementioned in the other scenarios are added another series of recommendations.
Continuous visualization and study of the teaching material provided for the exhibition classes, following temporal guidelines as if they were face-to-face classes. In other words, do not let topics accumulate without reviewing and studying in order to study everything at the end of the course. The conduct of the seminars, although telematic to be the case, will be scheduled according to the foreseeable calendar in a face-to-face setting, so that the subject must be brought up to date.
Maximum attention and dedication to the realization and delivery of the seminars since: i) part of the exhibition material is reviewed; ii) is also subject of examination; iii) the answer bulletins are evaluated (a significant percentage of the final grade); iiii) the type of questions and issues covered are very similar to some that will appear on the exam.
Study and analysis of the teaching material of the laboratory practices for the optimal realization of the memory of practices. Careful preparation of the practice report.
Frequent reading and reflection on the subject taught, necessary for the integration of knowledge.
COMMUNICATION WITH TEACHERS
There will be an active virtual classroom of the subject through the Virtual Campus of the USC.
Scenario 1 (adapted normality) and scenario 2 (distancing; partial restrictions on attendance). Face-to-face communication in the classroom and in the office. Communications may also be established through institutional email, via the MS-Teams platform, messages through the Virtual Campus forum and by telephone.
Scenario 3 (closure of the facilities; impossibility of teaching in person). Communication with teachers will be established through the institutional email and the Virtual Campus forum , via telephone and via the MS-Teams platform (the latter as long as the teaching staff can access their office).
Guillermo Covelo Artos
Coordinador/a- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816930
- guillermo.covelo [at] usc.es
- Category
- Professor: Temporary PhD professor
| Monday | |||
|---|---|---|---|
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Main Hall Santiago Ramón y Cajal |
| Tuesday | |||
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Main Hall Santiago Ramón y Cajal |
| Wednesday | |||
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Main Hall Santiago Ramón y Cajal |
| 05.18.2022 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 05.18.2022 10:00-14:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
| 05.18.2022 10:00-14:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |
| 07.05.2022 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |