ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.25 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.5
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology, Organic Chemistry
Areas: Biochemistry and Molecular Biology, Organic Chemistry
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
• List the most common strategies and methods in protein engineering.
• Identify the practical applications of protein engineering in biotechnology.
• Distinguish the methods of representing biomolecular structures and know how to use the software for their analysis.
Identify the factors that influence protein folding.
Regular classes
• Introduction. Protein engineering applications: food industry, detergents, environmental applications, medical applications, biopolymers, nanobiotechnological applications.
• Protein folding: Thermodynamics and folding kinetics. In vivo folding.
• Protein determination methods. Structural databases and software for representation of biomolecule structures.
• Design and synthesis of de novo proteins: Rational methods, incorporation of unnatural amino acids, assembly of tertiary structures from elements of secondary structure, etc. Bioconjugation.
• Representative cases of modified proteins. Oxidation resistant proteases, Insulins, TPA, GFPs, FDH, etc.
Seminars
• Real case analysis of designed peptides and proteins with various applications.
Computer classes
• Representation and study of biomolecule structures. Use of the UCSF Chimera and/or PyMol program. Use of structural databases (PDB); Molecular design servers (Rosie).
Group tutoring
• Reinforcing the subject through presentations or discussion of related topics.
• V. Köhler (Ed.) Protein Design, Metods and Applications in Methods in Molecular Biology, 2014 Humana Press.
• K. J. Jensen (Ed.). Peptide and Protein Design for Biopharmaceutical Applications, 2009 John Wiley & Sons.
• E Buxbaum, Fundamentals of Protein Structure and Function, 2007 Springer.
• C. Köhrer, U. L. RajBhandary (Eds.). Protein Engineering in Nucleic Acids and Molecular Biology series no. 22, 2009, Springer.
• P. Kaumaya, Protein Engineering, 2012, InTech.
Basic and General
CG1 - Know the most important concepts, methods, and results of the different branches of Biotechnology.
CG2 - Apply the theoretical-practical knowledge acquired in the approach to problems and the search for solutions in both academic and professional contexts.
CG3 - Know how to obtain and interpret relevant information and results and draw conclusions on issues related to Biotechnology.
CG4 - Be able to transmit information both in writing and orally and to discuss ideas, problems, and solutions related to Biotechnology, before the general or specialized public.
CG5 - Study and learn autonomously, with the organization of time and resources, new knowledge and techniques in Biotechnology and acquire the ability to work as a team.
CB1 - That the students have demonstrated to possess and understand knowledge in an area of study that begins at the base of general secondary education and is usually found at a level that, although supported by advanced textbooks, also includes some aspects involving knowledge from the forefront of their field of study
CB2 - That students know how to apply their knowledge to their work or vocation in a professional way and possess the competencies that are usually demonstrated through the elaboration and defense of arguments and the resolution of problems within their area of study
CB3 - That the students before them have the ability to collect and interpret relevant data (usually within their area of study) to make judgments that include a reflection on relevant issues of a social, scientific or ethical nature
CB4 - That students can transmit information, ideas, problems and solutions to an audience both specialist and non - specialist
CB5 - That the students have developed those learning skills necessary to undertake further studies with a high degree of autonomy .
Transversal
CT1 - Think in an integrated way and approach problems from different perspectives.
CT2 - Search, process, analyze, and synthesize information from various sources.
CT3 - Organize and plan your work.
CT4 - Interpret result s experimental and identify consistent and inconsistent elements.
CT7 - Maintain an ethical commitment.
CT6 - Critical reasoning.
Specific
CE2 - Understand and know how to apply the physical-chemical principles of two biological processes with application in Biotechnology, as well as the main tools used to investigate them.
CE3 - Know how to apply the instrumental techniques and work protocols in a laboratory, applying the regulations and techniques related to safety and hygiene, waste management and quality.
Adapted in accordance with the "Bases para o desenvolvemento dunha docencia presencial segura no curso 2020-2021” and with the “ Directrices para os desenvolvemento dunha docencia presencial segura, curso 2020-2021” . Three scenarios are contemplated for the purposes of teaching safe: and scenario 1 : normally adapted (unrestricted physical contact) ; and scenario 2: social distancing (with partial restrictions to physical contact) ; and scenario 3: closing l installation as ones (inability to provide contact teaching).
Classes and exhibitions. The teachers develop to the content of the program using exercises and practical examples that illustrate the concepts explained in combination with additional resources and the recommended bibliography. In stage 1 these will be in classroom teaching and/or hybrid teaching using MS Teams. In any case, this will be done minizing physical contact and following the safety recommendations from the authorities (e.g., use of a mask ); in scenarios 2 and 3 classes will be online using the MS Teams platform.
Seminars and tutoring. Real cases of peptide and protein design will be analyzed in the classroom. Students are encouraged to actively participate in the classroom activities throughout the entire course; participation one of the criteria of evaluation. Students will be given handouts with problems to try to resolve on their own, and give their proposed solution to teachers in advance of the seminars. In these classes, the students will present their solutions, the teacher being in charge of solving the doubts and difficulties that arise. Occasionally, the seminars will also propose short exercises to solve in the moment, which will be taken into account in the evaluation. The interactive classes will perform án classroom in scenario 1 and through MS Teams and Moodle platforms in phases 2 and 3.
Individual tutoring. This will involve close work with the students to meet their needs. they will be carried out in person or online depending on the scenario in which we find ourselves.
Computer lab. Two sessions s in which the students will familiarize themselves with various tools used for representing biomolecules, databases relevant and software to design proteins (Rosie)
Virtual classroom (Moodle). A virtual classroom in will contain all the material support teaching of the course, calendars, links, etc. It will be the means to be used to carry out the final tests in scenario 3. This platform also contains discussion forums and internal mail, which provides excellent communication between teachers and students.
MS Teams. MS Teams will be used for the online classes and generally for voice and video communications and students and teachers.
The overall grade of the student in the subject will result from making a weighted average between the scores obtained for their performance in the final exam (50%), and the grade derived from the continuous evaluation of the work in the seminars and tutorials (50%). These same instruments will be used to evaluate the subject's competences, with particular emphasis on the specific competencies of the subject.
Final exam: Scenario 1 final exam face-to-face; in scenario 2 the final exam will be preferably virtual and in scenario 3 there will be a telematic final exam on the platforms of the virtual Campus (Moodle) and/or MS Teams. The final test will be carried out using resources that guarantee the identification of the student and the personal nature of the test. In the event that the means that guarantee compliance with these premises are not available, an oral final test (MS Teams) will be used.
Continuous evaluation: In scenario 1, the continuous evaluation will be carried out by combining face-to-face activities, such as teamwork, presentations or solving questions in class, with online activities using the resources of institutional platforms (Virtual Classroom-Moodle and Microsoft Teams); in scenarios 2 and 3 the activities will be telematic.
In cases of fraudulent performance of exercises or tests, the provisions of the “Regulations for the assessment of two academic performance and the review of qualifications” will apply.
Student work 75 h
It is important to highlight that the student's personal work has to be continued throughout the subject, since it is a subject in which the contents that are taught as the program progresses are based on the knowledge acquired in the previous topics. .
Contingency plan: changes in the teaching methodology and assessment methods are contemplated depending on the evolution of the SARS-Cov2 pandemic and the changes between scenarios 1, 2 and 3.
Jose Manuel Martinez Costas
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881815734
- jose.martinez.costas [at] usc.es
- Category
- Professor: University Professor
Marco Eugenio Vazquez Sentis
Coordinador/a- Department
- Organic Chemistry
- Area
- Organic Chemistry
- Phone
- 881815738
- eugenio.vazquez [at] usc.es
- Category
- Professor: University Lecturer
Jacobo Gómez González
- Department
- Organic Chemistry
- Area
- Organic Chemistry
- Category
- Ministry Post-doctoral Contract
Eva Rivera Chao
- Department
- Organic Chemistry
- Area
- Organic Chemistry
- eva.rivera [at] usc.es
- Category
- Xunta Pre-doctoral Contract
| Monday | |||
|---|---|---|---|
| 11:00-12:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
| Tuesday | |||
| 11:00-12:00 | Grupo /CLE_01 | Spanish | Virtual classroom |
| 06.11.2021 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 06.11.2021 10:00-14:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
| 07.22.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 07.22.2021 16:00-20:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |