ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Student's work ECTS: 99 Hours of tutorials: 3 Expository Class: 24 Interactive Classroom: 24 Total: 150
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology
Areas: Biochemistry and Molecular Biology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
- To identify the methods and applications of recombinant DNA technology.
- To identify the most used techniques and strategies for the production of recombinant proteins.
- To number gene-editing and genome sequencing technologies.
- To identify and apply experimental protocols for the management and analysis of nucleic acids.
-To know how to clone and express recombinant proteins.
Lectures and interactive seminar classes (27 h + 9 h)
BLOCK I. ESSENTIALS TOOLS AND BASIC TECHNIQUES (9 h)
Unit 1. Introduction to Genetic engineering (1 h)
Unit 2. Enzymology of recombinant DNA (3 h)
Unit 3. Basic techniques for isolation, purification and analysis of nucleic acids (2.5 h)
Unit 4. PCR (2.5 h)
BLOCK II. MOLECULAR CLONING (9 h)
Unit 5. Introduction to molecular cloning (2.5 h)
Unit 6. Molecular cloning in bacterial cells (2.5 h)
Unit 7. Libraries (2 h)
Unit 8. Molecular cloning in eukaryotic cells (2 h)
BLOCK III. DNA MODIFICATION AND EXPRESSION OF RECOMBINANT DNA (9 h)
Unit 9. Expression of recombinant DNA (4 h)
Unit 10. Site-directed mutagenesis. Introduction to protein engineering (2 h)
Unit 11. Gene inactivation and gene editing. Transgenic organisms. (3 h)
Seminars
-Seminars 1 and 2: problems and questions concerning units 2 and 3. (3 h)
-Seminar 3: management of databases and computer programs. (2 h)
-Seminar 4: problems and exercises related to units 5-7. (2 h)
-Seminar 5: presentation of a work that will cover topics explained throughout the course. (2 h)
Each student will have four work sessions in which they will perform:
-Session 1: Cloning of cDNA amplified by PCR. Transformation of E. coli cells and selection of transformants. (4 h)
-Session 2: Plasmid purification, digestion with restriction enzymes and analysis in agarose electrophoresis. (3 h)
-Session 3. Expression and purification of a recombinant protein. (2 h)
-Session 4: Analysis of the recombinant protein in polyacrylamide gel electrophoresis. Interpretation of the results. (2 h)
Basic bibliography
-Brown, T.A., 2016. Gene cloning and DNA analysis: An Introduction. 7th ed. Chichester: Wiley-Blackwell.
-Clark, D.P. and Pazdernik, N.J., 2016. Biotechnology. [en liña] 2nd ed. Amsterdam: Elsevier Academic Press. Dispoñible en: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
-Perera, J., Tormo, A. y García, J.L., 2002. Ingeniería genética. 2 v. Madrid: Síntesis.
-Real García, M.D., Rausell Segarra, C. y Latorre Castillo, A., 2017. Técnicas de ingeniería genética. Madrid: Síntesis.
Complementary bibliography
-Clark, D.P, 2019. Molecular Biology. [en liña] 3rd ed. Amsterdam: Elsevier . Dispoñible en: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128132883/molecul…
-Glick, B.R. and Patten, C.L., 2017. Molecular biotechnology: principles and applications of recombinant DNA. 5th ed. Washington DC: ASM Press.
-Green, M.R. and Sambrook, J., 2012. Molecular cloning: a laboratory manual. 4th ed. 3 v. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
-McPherson, M.J. and Møller, S., 2006. PCR. 2nd ed. New York: Taylor & Francis.
-Renneberg, R., Berkling, V., and Loroch, V., 2016. Biotechnology for beginners [en liña]. Amsterdam: Elsevier. Dispoñible en: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128012246/biotech…
Others resources
- http://biomodel.uah.es. Web site of the Universidad Alcalá de Henares
-https://www.jove.com/science-education-library. Educational videos educativos about different molecular biology and genetic engineering techniques.
- http://www.ncbi.nlm.nih.gov/guide/. The National Center for Biotechnology Information, (NCBI) main source of data Banks (nucleotides, genes and genomes) and bioinformatics applications.
-Electronic resources from BUSC: Cell, Nature, Science, Sciencedirect, etc.
BASIC AND GENERAL COMPETENCES (CB and CG)
As indicated in the report of the Grade in Biotechnology.
TRANSVERSAL SKILLS
CT1 – Students should think in an integrated way and approach problems from different perspectives.
CT2 – To search, manage, analyse and synthesise information from different sources.
CT4 – Interpret experimental results and identify consistent and inconsistent elements.
CT5 – To work in group.
CT6 – Critical reasoning.
CT7 – Students must maintain an ethical commitment.
SPECIFIC SKILLS
CE11– To know the molecular basis and techniques for manipulating gene information in microorganisms, animals and plants and to know how to properly apply these techniques in different biotechnological fields.
The general methodologies of the degree described in section 5.3 of the report will be followed. Thus, the following methodologies will be used:
- MASTER LECTURES: will be held face-to-face; the techaer, through a master lesson and with the support of slides and course material available on the virtual campus, will explain de fundamental issues of the subject. Non-compulsory activity
- SEMINARS face-to-face in small groups, where the students, either individually or in small groups, will solve problems or issues. The students will also prepare a topic in small groups and also could discuss topics related to the subject. Non-compulsory activity
- TUTORIALS in very small groups for the clarification of doubts about the theory or practices, to provide information or guide the students. The first one will be face-to-face, the others will be held electronically. Non-compulsory activity
- LABORATORY PRACTICAL CLASSES, in which the students, following the protocols prepared for this purpose, will handle the appropriate equipment and solve practical questions. Each student will have four work sessions in the laboratory. Mandatory activity.
1. The evaluation will consist of two parts:
1.1) Continuous evaluation (30% of the final grade), which in turn consists of:
i. Seminars (20% of the final grade). NON-MANDATORY ACTIVITY THAT CAN BE EVALUATED.
The evaluation will be based on the exercises / reports presented to the teacher or carried out in the seminars, and to the active participation in the academic activities. The lack of attendance at a minimum of 80% of the seminar hours implies the waiver of the right to seminars evaluation.
ii. Laboratory practices (10% of the final grade). EVALUABLE MANDATORY ACTIVITY.
For the grade, a final report and/or questions will be scored. Unexcused absences will imply a qualification of NOT SUITABLE. Students must obtain a qualification higher than 4 in the practices to overcome the subject.
The control of attendance to face-to-face activities will be by signature. Participation in telematic activities will be automatically registered in the on-line Campus and Microsoft Teams.
1.2) Final Test (70% of the final grade). EVALUABLE MANDATORY ACTIVITY, COMPLEMENTARY TO THE CONTINUOUS EVALUATION.
It will consist of questions / exercises related to the theoretical and practical content of the subject.
In scenario 1 it will be face-to-face and will consist in cuestions/exercises related to the theoretical and practical contents of the subject.
The final qualification of the student will be obtained by weighing the final test with the continuous assessment (it is necessary to have a minimum score of 4 out of 10 points in the final exam to do the weighing). Otherwise, the final grade will be exclusively that of the exam (60% of the total grade). A minimum final grade of 5 out of 10 will be necessary to pass the course.
2. Students who fail the course in the ordinary exam opportunity must retake the final test during the recovery opportunity (2nd opportunity). Scores obtained for the continuous evaluation will be maintained for the recovery opportunity. Those students who suspend the practices must do a recovery test.
3. Students who do not pass the subject but who have passed the laboratory practices will maintain those marks for the next academic year. Students who are enrolled in the class for the second time or more will be evaluated following the same guidelines for attendance to classes and calculating the final grade as students enrolled in the class for the first time.
In the final exam the competences will be evaluated: CB1/2/5, CG1/4/5, CT2/6, CE11.
In the seminars: CB1/2/3/4, CG1/3/4/5, CT1/2/5/6, CE11.
In the practices: CB2/4/5, CG1/3, CT4/5/7, CE11.
In cases of fraudulent carrying out of exercises or tests, the provisions of the "Student Assessment and Academic Performance Assessment Regulations" of the USC will apply.
In general, an average of 150 hours of work is estimated for this subject, of which 51 hours correspond to attendance at theoretical and practical classes, seminars and tutorials, 96 hours to self-employment of the student and the remaining 3 to the performance of exams and review of them.
- Assistance and participation in the proposed activities, as well as bringing the matter up to date.
-Consult of the recommended bibliography.
-Use of the virtual classroom.
-To make use of the tutorials for any type of matter related to the subject.
For a better understanding of this subject, the student must have knowledge of Biochemistry I and first topics of Biochemistry II, Genetics I and Genetics II (gene expression).
There will be an operational virtual classroom in the Moodle platform with material related to classes, seminars and practices. Announcements will also be made through this platform, so students should check their institutional e-mail frequently.
The preferred channel of communication will be e-mail. If necessary, communication can be established through MS-Teams.
IT IS EXPRESSLY FORBIDDEN FOR STUDENTS TO DISTRIBUTE TEACHING MATERIAL (BOTH WRITTEN AND AUDIOVISUAL) TO PEOPLE OUTSIDE THE COURSE.
Cristina Diaz Jullien
Coordinador/a- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816932
- cristina.diaz [at] usc.es
- Category
- Professor: Temporary PhD professor
Guillermo Covelo Artos
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816930
- guillermo.covelo [at] usc.es
- Category
- Professor: Temporary PhD professor
Laura Rivadulla Costa
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- laura.rivadulla.costa [at] usc.es
- Category
- Ministry Pre-doctoral Contract
| Monday | |||
|---|---|---|---|
| 16:00-17:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| Wednesday | |||
| 17:00-18:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| 05.16.2023 16:00-20:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 07.03.2023 16:00-20:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |