
Mercedes Novo Rodríguez
Biographical sketch
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1982-1987 Degree in Chemical Sciences (Physical Chemistry) at the USC. Bachelor's thesis.
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1991 PhD Thesis "Photoinduced proton transfer processes in 2-pyridylbenzimidazoles", Department of Physical Chemistry, USC, Directors: Profs. Flor Rodríguez Prieto and Manuel Mosquera González.
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1988 Predoctoral stay at the Center for Structural Chemistry of the Higher Technical Institute of Lisbon (Portugal), under supervision of Prof. Antonio Maçanita.
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1990 Predoctoral stay at the Spectroscopy Department of the Max-Planck-Institut für biophysikalische Chemie in Göttingen (Germany), under supervision of Dr. Bernhard Nickel and Dr. Karl H. Grellmann.
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1992 Postdoctoral stay at the Laboratory of Molecular Dynamics and Spectroscopy of the Katholieke Universiteit Leuven (Belgium), under supervision of Prof. Frans C. De Schryver.
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1992-1995 Assistant Professor at the USC.
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1993-1996 Postdoctoral stays and research-contract in the photobiology group of the Medical Laser Center in Lübeck (Germany), with Dr. Heyke Diddens and Prof. Reginal Birngruber.
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Since 1996, Associate Professor at the Department of Physical Chemistry of the USC in Lugo (permanent position in 1998).
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Since 2003, together with Prof. Wajih Al-Soufi, director of the Single Molecule Fluorescence Research Unit of the Group Biophysical Chemistry, Photophysics and Spectroscopy (USC).
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2015 National Accreditation as Full Professor (Acreditación National para Catedrático de Universidad), 3th of november, 2015.
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Since 2013 Founder and Chief Coordinator of the “Teaching Innovation Group XuvenCiencia” http://www.usc.es/xuvenciencia(link is external). “XuvenCiencia”, offers training to school staff, develops educational science kits and organizes one of the biggest annual scientific summer campus for young people in Spain.
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2023 Full Professor of Physical Chemistry
I received my training in physical chemistry and especially in photophysics under the direction of Professors Flor Rodríguez Prieto and Manuel Mosquera González and during several stays in laboratories specialized in fluorescence life-time measurements, flash photolysis and phosphorescence techniques applied to systems with photoinduced proton transfer. In postdoctoral stays at the Catholic University of Louvain (Belgium), I worked with Langmuir Blodgett films and the "time of flight" technique, and at the Medical Laser Center in Lübeck (Germany) I conducted studies on the intracellular location of different photosensitizers used for the diagnosis and treatment of cancer in the field of photodynamic therapy (PDT).
In 2003, together with Prof. Dr. Wajih Al-Soufi, I founded the Single Molecule Fluorescence Research Unit for the application of fluorescence microscopy techniques of high spatial and temporal resolution to systems of biological or technological interest such as DNA, proteins, liposomes, micelles or cyclodextrins. Our main interest is the study of the dynamics of the supramolecular association and the processes of biomolecular recognition. For this purpose, we apply fluorescence correlation spectroscopy (FCS), multiparametric fluorescence detection of individual molecules (MFD) and internal reflection fluorescence microscopy (TIRF).
In 2004 we presented for the first time the application of FCS to the dynamics of host-guest-type molecular inclusion processes (JACS 2005, 127, 8775) and to the dynamics of the solubilization in micelles (JPC B, 2007, 3614). We determined the sequence specificity of the binding dynamics of minor groove ligands of DNA (Angew, Chem. Int. Ed., 2012, 51, 7541) and we recently published the first direct determination of the critical aggregation concentration for the formation of early Amyloid-β (1-42) oligomers responsible for Alzheimer’s disease (Scientific Reports 2018, 8.). Since 2004, we have obtained continuous funding in competitive calls and participated in national research networks and EU-COST actions.
Databases: Web Grupo, USC, ResearchGate, ORCID, Google Scholar, LinkedIn, Scopus
Publications
CRISPR/Cas9 Delivery Systems to Enhance Gene Editing Efficiency
Amyloid Capture and Aggregation Inhibition by Human Serum Albumin
International Journal of Biological Macromolecules, Volume 301, April 2025, 140367
https://doi.org/10.1016/j.ijbiomac.2025.140367
(Preprint: ChemRxiv, 2024, Version 1, 10.26434/chemrxiv-2024-fr8gt)
Thiazole Orange as fluorescent marker for the detection and quantification of dsDNA
ChemRxiv, 2024, Version 1, Preprint, 10.26434/chemrxiv-2024-lbxzx
Critical aggregation concentration and reversibility of amyloid-β (1–40) oligomers
Archives of Biochemistry and Biophysics, 2024, 761, 110179, DOI 10.1016/j.abb.2024.110179
Methods in Molecular Biology, 2023, 2551, 1–14. DOI: 10.1007/978-1-0716-2597-2_1
Frontiers in Chemistry 2022, 10, 1042658. DOI: 10.3389/fchem.2022.1042658 (Open Access)
International Journal of Molecular Sciences 2022, 23(2), 925. DOI: 10.3390/ijms23020925 (Open Access)
Molecules 2021, 26, no. 17: 5339. https://doi.org/10.3390/molecules26175339 (Open Access)
Journal of Chemical Education 2020, 97, 9, 3090–3096, https://doi.org/10.1021/acs.jchemed.0c00745
Scientific Reports 2019, 9, Article number: 12897, DOI: 10.1038/s41598-019-49210-0 (Open Access)
Scientific Reports 2018, 8, Article number: 1783, DOI: 10.1038/s41598-018-19961-3 (Open Access)
Chemistry - A European Journal 2015, 21, 1609–1619. DOI: 10.1002/chem.201404926
Chemistry - A European Journal 2015, 21, 3425–3434. DOI: 10.1002/chem.201406110
Advances in Colloid and Interface Science 2015, 215, 1–12. DOI:10.1016/j.cis.2014.10.010
Nanomedicine : Nanotechnology, Biology, and Medicine 2014, 10 (4), 851–858, DOI: 10.1016/j.nano.2013.12.004)
Dyes and Pigments 2014, 110, 97-105
DOI 10.1016/j.dyepig.2014.05.004 Accepted Author Manuscript (AAM)
Soft Matter 2013, 9, 10779-10790, DOI: 10.1039/ c3sm52092g (pdf)
Angewandte Chemie International Edition 2012, 51, 7541–7544
Journal of Colloid and Interface Science 2012, 370, 102–110 DOI: 10.1016/j.jcis.2011.12.037 (pdf) (Supplementary Information)
in Reviews in Fluorescence, volume 2009, 2011, 6, 23–45, ed. Chris D. Geddes, Springer, New York
Journal of Inclusion Phenomena and Macrocyclic Chemistry 2011, 70, 259-268
International Journal of Molecular Sciences 2010, 11, 173–188
Journal of Colloid and Interface Science 2010, 345, 369–376
Photochemical & Photobiological Sciences 2010, 9, 687–696
ChemPhysChem 2009, 10, 931–939
ChemPhysChem 2008, 9, 1819–1827
Journal of Physical Chemistry B 2007, 111, 3614-3624
Journal of Chemical Education 2007, 84, 1673-1675
Journal of the American Chemical Society 2005, 127, 8775-8784
Journal of Physical Chemistry B 2005, 109, 1364-1370
Supramolecular Chemistry 16, 549-559 (2004)
Journal of Agricultural and Food Chemistry 51, 5036-5040 (2003)
Journal of Agricultural and Food Chemistry 50, 108-112 (2002)
Applied Spectroscopy 2001, 55, 630–636
Influence of pH on the stability of the inclusion complex of methyl orange and beta-cyclodextrin
Proceedings of the 9th International Symposium on Cyclodextrins, 1998, Santiago de Compostela, Spain.
Kluwer Academic Publishers, Dordrecht, 179-182 (1999)
Chemical instability of 5-aminolevulinic acid used in the fluorescence diagnosis of bladder tumors
Journal of Photochemistry and Photobiology, B: Biology 34, 143-148 (1996)
Journal of Physical Chemistry 1995, 99, 14726–14732
Journal of the Chemical Society, Faraday Transactions 1993, 89, 885–889
Photoinduced electron transfer in and electron injection from trichromophoric molecules forming Langmuir Blodgett films
Molecular Crystals and Liquid Crystals Science and Technology, Section A: Molecular Crystals and Liquid Crystals 235, 67-74 (1993)
Anomalous field dependence of the hole mobility in a molecular doped polymer
Physica Status Solidi B: Basic Research 177, 223-41 (1993)
Tautomerism - to be taken lightly
Education in Chemistry 1992, 29, 139–141
Canadian Journal of Chemistry 1992, 70, 823–827
Journal of Physical Chemistry 95, 10509-10518 (1991)
Journal of Physical Chemistry 1990, 94, 8536–8542